Visualization of Metabolites Identified in the Spatial Metabolome of Traditional Chinese Medicine Using DESI-MSI

1.7K Views

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08:02 min

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December 16th, 2022

DOI :

10.3791/64912-v

December 16th, 2022

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Binjie Xu¹^,⁴, Lirun Chen³, Fengqi Lv³, Yuan Pan¹, Xing Fu¹, Zhaoqing Pei¹^,²

¹Innovative Institute of Chinese Medicine and Pharmacy, Chengdu University of Traditional Chinese Medicine, ²Tianfu Chinese Medicine Innovation Harbour, Chengdu University of Traditional Chinese Medicine, ³School of Medical Technology, Chengdu University of Traditional Chinese Medicine, ⁴China Resources Sanjiu (Ya’an) Pharmaceutical Co., Ltd.

Summary

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Spatial Metabolomics

DESI MSI

Traditional Chinese Medicine

Toxicants Distribution

Abiotic Stress

Biotic Stress

Electrospray Ionization

Solvent Ionization

Mass Calibration

Chemical Analysis

Chapters in this video

0:04

Introduction

1:02

Sample Preparation

1:55

Installation of Desorption Electrospray Ionization (DESI) Unit

4:02

Desorption Electrospray Ionization‐Mass Spectrometry (DESI‐MS) Image Acquisition

5:50

Processing DESI‐MSI Data and Visualization

6:55

Results: Mass Spectrometry Imaging of Root and Whole Leaf Sections

7:31

Conclusion

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