We thank Junming Yan, consultant of Johnson&#38;Johnoson, who is responsible for research grants.This work was funded by the Jilin Provincial Department of Science and Technology (20220402076GH).

This research procedure closely adhered to the rules of human research ethics committee of China-Japan Union Hospital of Jilin University. Patients who underwent radiofrequency ablation of atrial fibrillation were searched on the Carto system (3D mapping system). Then, the PACS system was used to determine whether the patient underwent left atrial CT examination before surgery to ascertain that each selected patient had left atrial CT images for comparison. Soundstar is the ICE catheter used in this study, and a Cartosound module is available in the 3D mapping system. Each patient provided written informed consent before ICE 3D modeling.
1. Preparation before scanning
<ol>
	<li>Check patient information, such as accurate heart rate, rhythm, blood pressure, and oxygen saturation. Let the patient be supine with the arms on both sides and the thighs slightly abducted.</li>
	<li>Provide deep conscious sedation with fentanyl (200 &#181;g/mL) to all patients throughout the procedure. Select the right femoral vein as the puncture point, which is disinfected and paved for local anesthesia with 2% lidocaine.</li>
	<li>Set up the ICE catheter: Connect the ICE catheter tail line with the 3D mapping system and an ultrasonic machine. Open the Study interface in the 3D mapping system and select P500 in Connected Machines.</li>
	<li>Place the ICE catheter (Diameter: 10f; ultrasound mode: B-mode) inside the femoral vein. During the pushing process, monitor the ultrasound in real-time to ensure that the catheter is in a safe space.</li>
	<li>Pass the ablation catheter to the right atrium to develop the right atrium and coronary sinus model. Place the coronary sinus electrode by combining it with ICE guidance.</li>
	<li>After the ICE catheter enters the right atrium, ensure that the left atrium and left atrial appendage structure are displayed on the 3D mapping system display screen using the short and long axes of the left atrium while excluding the left atrial thrombus.</li>
	<li>Determine the optimal puncture site under ICE guidance after inserting the atrial septal puncture needle. Then, perform transseptal puncture.
	<ol>
		<li>Use the Home view to confirm that the ICE reached the right atrium. Then the software displays the left PV section. Click Right Bend to demonstrate the inferior vena cava and atrial septum.</li>
		<li>Move the puncture needle sheath to point in the four o&#39;clock direction. Retract the needle sheath to the oval fossa while monitoring ICE, and the &#34;tent sign&#34; is visible.</li>
		<li>Adjust the ICE catheter to reveal the left PV as a &#34;rabbit ear sign.&#34; Under ICE guidance, slowly withdraw the needle sheath to the lower edge of the oval fossa.</li>
		<li>Rotate the puncture needle clockwise to break through the oval fossa. Then, inject heparin saline through the atrial septum puncture needle. 
		&#8203;NOTE: Salt blisters were observed in the LA, indicating successful puncture of the atrial septum.</li>
	</ol>
	</li>
</ol>
2. 3D modeling of the left atrium and pulmonary vein
NOTE: The ICE constructs the left atrium model in two directions.
<ol>
	<li>After pushing the ICE catheter into the femoral vein, pass it through the inferior and superior vena cava and enter the right atrium.</li>
	<li>Push the ultrasound catheter to the middle of the right atrium on the short axis and rotate clockwise. Now, the ultrasound fan points toward the one o&#39;clock (Home view) direction depicting the right atrium and the right ventricle.</li>
	<li>Tighten the Tension Knob to achieve locking tension tuning. Then turn to the Home view and click Antecurvature (A) to fully display the tricuspid annulus. Attach to the tricuspid annulus for gating training and use the end-breath phase modeling.</li>
	<li>From the Home view, turn clockwise to the left atrial anterior wall, leading to the appearance of the left atrial appendage.</li>
	<li>Continue the clockwise turn to the left atrium, leading to the appearance of the left upper and lower pulmonary veins, displayed as a &#34;rabbit ear sign.&#34; Then, turn it clockwise and counterclockwise to precisely gather the image of the left pulmonary veins by identifying the anterior and posterior venus boundaries.</li>
	<li>Continue to turn clockwise to the left atrium, establishing the posterior wall, and in the process, the esophagus appears as the &#34;double track sign.&#34;</li>
	<li>Turn to the left atrium along the clockwise direction to observe the right lower pulmonary vein, displayed as a &#34;3-word sign&#34;. Then, turn it clockwise and counterclockwise to precisely capture the image of the right pulmonary veins by identifying the anterior and posterior boundaries.</li>
	<li>On the long axis, click Palintrope (P) to make the tip of the catheter at the same height as the coronary sinus mouth. This supplements the left atrium model. Adjust Left Bend/Right Bend (L/R) to observe the front wall of the long axis of the left atrium. This image captures the anterior wall of the left atrium.</li>
	<li>Mark the important anatomical locations, including pulmonary vein ostia, left atrial appendage, and other vital sites accordingly (Video 1).</li>
</ol>
3. Image acquisition and measurement of the pulmonary vein area
<ol>
	<li>Left atrial CT
	<ol>
		<li>Open the PACS system by double-clicking the icon. Click on Advanced Query to enter the patient name and inspection item. Click OK to find the image.</li>
		<li>Click on Tune to transfer the image to the vue pacs system (picture archiving and communication system).</li>
		<li>Transfer the 3D volume reconstruction image to the work box and click Export Image to save the left atrial posterior anterior (PA), left later (LL), and right lateral (RL) position images within the folder.</li>
		<li>After returning to the previous program, transfer the left atrial arterial phase enhancement sequence to the work box, and click on the image displayed as 3D.</li>
		<li>Double-click on the 3D image, then click on 3D in the toolbar. Select the Resection Tool to remove the ribs, spine, aorta, and other structures to expose the left atrium and pulmonary vein system.</li>
		<li>Expose the pulmonary vein vestibule. Click on Figure in the toolbar and select Area to calculate the cross-sectional area of the pulmonary vein vestibule.</li>
	</ol>
	</li>
	<li>ICE
	<ol>
		<li>Open the 3D mapping system. Then, click on Review Study and enter the name of the patient. Finally, use Search for Current Patient to identify the image.</li>
		<li>Click on OK to open the work interface.</li>
		<li>Click on Study &#62; Continue Study and select the Model and Channel sequences.</li>
		<li>Click on Capture Preferences, then select Region and adjust the image to &#34;Posterior-anterior&#34;, &#34;Left lateral,&#34; &#34;Right lateral,&#34; &#34;Left anterior oblique (LAO),&#34; and &#34;Right anterior oblique (RAO).&#34;</li>
		<li>Click on Picture, select the photo area, and click OK to save the picture.</li>
		<li>Click on the Map option and select Save Map. Then, use Eraser in the toolbar to remove the left and right pulmonary veins.</li>
		<li>Click on the image, select Area Measurement and measure the area of the pulmonary vein vestibule.</li>
	</ol>
	</li>
</ol>

ICE-Based 3D Remodeling for the Left Atrium and Pulmonary Vein

<ol>
	<li>Xu, Y., Sharma, D., Li, G., Liu, Y. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Atrial+remodeling:+new+pathophysiological+mechanism+of+atrial+fibrillation.">Atrial remodeling: new pathophysiological mechanism of atrial fibrillation.</a> Medical Hypotheses. 80 (1), 53-56 (2013).</li><li>George, J. C., Varghese, V., Mogtader, A. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Intracardiac+echocardiography:+evolving+use+in+interventional+cardiology.">Intracardiac echocardiography: evolving use in interventional cardiology.</a> Journal of Ultrasound in Medicine. 33 (3), 387-395 (2014).</li><li>Jingquan, Z., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Intracardiac+echocardiography+Chinese+expert+consensus.">Intracardiac echocardiography Chinese expert consensus.</a> Frontiers in Cardiovascular Medicine. 9, 1012731 (2022).</li><li>Anter, E., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Comparison+of+intracardiac+echocardiography+and+transesophageal+echocardiography+for+imaging+of+the+right+and+left+atrial+appendages.">Comparison of intracardiac echocardiography and transesophageal echocardiography for imaging of the right and left atrial appendages.</a> Heart Rhythm. 11 (11), 1890-1897 (2014).</li><li>Rossillo, A., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Novel+ICE-guided+registration+strategy+for+integration+of+electroanatomical+mapping+with+three-dimensional+CT/MR+images+to+guide+catheter+ablation+of+atrial+fibrillation.">Novel ICE-guided registration strategy for integration of electroanatomical mapping with three-dimensional CT/MR images to guide catheter ablation of atrial fibrillation.</a> Journal of Cardiovascular Electrophysiology. 20 (4), 374-378 (2009).</li><li>Okumura, Y., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Three-dimensional+ultrasound+for+image-guided+mapping+and+intervention:+methods,+quantitative+validation,+and+clinical+feasibility+of+a+novel+multimodality+image+mapping+system.">Three-dimensional ultrasound for image-guided mapping and intervention: methods, quantitative validation, and clinical feasibility of a novel multimodality image mapping system.</a> Circulation: Arrhythmia and Electrophysiology. 1 (2), 110-119 (2008).</li><li>Liu, C. F. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=The+evolving+utility+of+intracardiac+echocardiography+in+cardiac+procedures.">The evolving utility of intracardiac echocardiography in cardiac procedures.</a> Journal of Atrial Fibrillation. 6 (6), 1055 (2014).</li><li>Bartel, T., Edris, A., Velik-Salchner, C., M&#252;ller, S. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Intracardiac+echocardiography+for+guidance+of+transcatheter+aortic+valve+implantation+under+monitored+sedation:+a+solution+to+a+dilemma.">Intracardiac echocardiography for guidance of transcatheter aortic valve implantation under monitored sedation: a solution to a dilemma.</a> European Heart Journal. Cardiovascular Imaging. 17 (1), 1-8 (2016).</li><li>Haissaguerre, M., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Spontaneous+initiation+of+atrial+fibrillation+by+ectopic+beats+originating+in+the+pulmonary+veins.">Spontaneous initiation of atrial fibrillation by ectopic beats originating in the pulmonary veins.</a> New England Journal of Medicine. 339 (10), 659-666 (1998).</li><li>Kaseno, K., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=The+impact+of+the+CartoSound&#174;+image+directly+acquired+from+the+left+atrium+for+integration+in+atrial+fibrillation+ablation.">The impact of the CartoSound&#174; image directly acquired from the left atrium for integration in atrial fibrillation ablation.</a> Journal of Interventional Cardiac Electrophysiology. 53, 301-308 (2018).</li><li>Enriquez, A., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Use+of+intracardiac+echocardiography+in+interventional+cardiology:+working+with+the+anatomy+rather+than+fighting+it.">Use of intracardiac echocardiography in interventional cardiology: working with the anatomy rather than fighting it.</a> Circulation. 137 (21), 2278-2294 (2018).</li></ol>

The authors have no conflicts of interest to declare.

Intracardiac echocardiography (ICE) is a noncontact three-dimensional reconstruction tool. It determines the appropriate ablation plane and reduces the incidence of pulmonary vein stenosis. Moreover, ICE improves the effectiveness of catheter ablation by assessing the distal position of the ablation catheter and its relative association with the anatomical structures. These structures include the left atrium and pulmonary vein and the diameter and morphology of the pulmonary vein.
ICE-guided a...

Atrial fibrillation (AF) is commonly associated with atrial remodeling, including mechanical remodeling, electrophysiologic remodeling, and structural remodeling1. Structural remodeling will dramatically affect the anatomy of the atrium. Hence, assessing the left atrial anatomy in AF patients is essential for AF ablation procedures and any procedure targeting the left atrium. For the FAM 3D modeling, the 3D modeling of the heart is reconstructed based on the spatial position change of its position corresponding to the fixed magnetic field by continuously displacing the magnetic catheter in the heart. In contrast, ICE 3D modeling integrates the two-dimensional image in the cardiac cavity with the 3D electroanatomic mapping system by positioning the sensor at the head end of the ICE phase array catheter. Thus, the ultrasonic sector represents 3D modeling to demonstrate the anatomical relationship and real-time catheter position.
Based on our clinical experience, intracardiac echocardiography (ICE) can identify the atrial wall boundary and further establish the 3D remodel. However, most ICE usage during AF ablation or 3D remodeling just provides a brief profile of the atria or pulmonary veins. Originally, ICE was applied to guide the interventional closure of atrial septal defect and patent foramen ovale2. ICE can clarify the location and shape of the atrial septal and has been used for various interventional procedures requiring atrial septal puncture3. These include radiofrequency catheter ablation of atrial fibrillation, mitral valve interventional therapy, etc. ICE can precisely identify pulmonary vein boundaries and atrial walls to establish a more detailed 3D model3. It is unclear whether this method could provide operators with a more precise atrial anatomy assessment, especially for the pulmonary vein antrum and transseptal sites. In this study, we compared the left atrium CT image and 3D remodel established using traditional methods and precise ICE procedures to determine additional information.

<table border="1" fo:keep-together.within-page="1" fo:keep-with-next.within-page="always">
	<tbody>
		<tr>
			<td>CARTO V6</td>
			<td>Johnson&#38;Johnson</td>
			<td>6.0.80.45</td>
			<td></td>
		</tr>
		<tr>
			<td>CARTO V7</td>
			<td>Johnson&#38;Johnson</td>
			<td>7.1.80.33</td>
			<td></td>
		</tr>
		<tr>
			<td>PACS system</td>
			<td>Philips(China) Investment Co.,Ltd</td>
			<td>N/A</td>
			<td></td>
		</tr>
		<tr>
			<td>Soundstar</td>
			<td>Johnson&#38;Johnson</td>
			<td>N/A</td>
			<td></td>
		</tr>
	</tbody>
</table>

three-dimensional modeling of the left atrium and pulmonary veins with a precise intracardiac echocardiography approach

Intracardiac echocardiography (ICE) is a novel tool for estimating cardiac anatomy during pulmonary vein isolation procedures, particularly the left atrium (LA) anatomy and pulmonary vein structures. ICE is widely used to establish a three-dimensional (3D) left atrial structural model during ablation procedures. However, it is unclear whether using ICE in a precise 3D modeling method can provide a more accurate left atrial 3D model and the transseptal approach. This study proposes a protocol to model the left atrium and pulmonary veins with ICE and fast anatomical mapping (FAM) catheter remodeling. It evaluates the accuracy of the models produced using the two methods through observer scoring. We included 50 patients who underwent ICE-based 3D remodeling and 45 who underwent FAM 3D remodeling based on pulmonary vein isolation procedures. The pulmonary vein antrum remodeling is estimated by comparing the antrum area acquired by remodeling and left atrial computed tomography angiography (CTA). The observer scores for the modeling in the ICE and FAM groups were 3.40 &#177; 0.81 and 3.02 &#177; 0.72 (P &#60; 0.05), respectively. The pulmonary vein antrum area obtained using the ICE- and FAM-based methods showed a correlation with the area acquired by left atrium CT. However, the 95% confidence interval bias was narrower in ICE-acquired models than in FAM-acquired models (-238 cm2 to 323 cm2 Vs. -363 cm2 to 386 cm2, respectively) using Bland-Altman analysis. Therefore, precise ICE possesses high accuracy in estimating the left atrial structure, becoming a promising approach for future cardiac structure estimation.

Author Spotlight: Advancements in Intracardiac Echocardiography for Atrial Anatomy Assessment 

Three-Dimensional Modeling of the Left Atrium and Pulmonary Veins with a Precise Intracardiac Echocardiography Approach

Our research tries to evaluate whether intracardiac echocardiography-based 3D construction could enhance the accuracy of pulmonary atrium and assessments in arterial anatomy. Intracardiac echocardiography is capable of identifying the arterial wall boundary and creating a 3D model of pulmonary winds. Recently this has been increased, the focus on using S2 often detailed images for accurately determining arterial anatomy.One of the current experimental challenges is accurately imitating the left arterial structure. However, promising results have been obtained use as best arterial imaging and safety deconstruction, which has high accuracy and hold potential for future cardiac structure imaging.

From January 2021 to June 2022, we selected 114 patients who underwent radiofrequency ablation of atrial fibrillation at our hospital. Patients were excluded based on the following criteria: no 3D volume reconstruction image of left atrial CT (n = 11), no transseptal puncture ICE image (n = 4), and incomplete reconstruction of the left atrium and pulmonary vein images (n = 4). Finally, 50 patients with ICE 3D modeling and 45 with FAM 3D modeling as control group was included in this study.
Two...

Watch this Scientific Journal Video about Advancements in Intracardiac Echocardiography for Atrial Anatomy Assessment at JoVE.com

Accurate intracardiac echocardiography (ICE) shows significant accuracy in estimating the left atrial structure, a prospective and promising cardiac structure estimation method. Here we propose a protocol for three-dimensional modeling of the left atrium and pulmonary veins with ICE and fast anatomical mapping (FAM) catheter remodeling.

Intracardiac echocardiography (ICE) is a novel tool for estimating cardiac anatomy during pulmonary vein isolation procedures, particularly the left ...

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581 Views.  China-Japan Union Hospital of Jilin University. Our research tries to evaluate whether intracardiac echocardiography-based 3D construction could enhance the accuracy of pulmonary atrium and assessments in arterial anatomy. Intracardiac echocardiography is capable of identifying the arterial wall boundary and creating a 3D model of pulmonary winds. Recently this has been increased, the focus on using S2 often detailed images for accurately determining arterial anatomy.One of the current experimental challenges is accurately imitating...

Video: Author Spotlight: Advancements in Intracardiac Echocardiography for Atrial Anatomy Assessment 

3D Modeling of the Left Atrium and Pulmonary Vein

Begin by inserting the intracardiac echocardiography catheter, or ICE catheter, into the femoral vein and guiding it through the inferior and superior vena cava into the right atrium. Position the ultrasound catheter in the middle of the right atrium on the short axis and rotate it clockwise, aiming the fan towards the one o'clock direction or home view to view the right atrium and right ventricle. Next, tighten the tension knob to achieve locking tension tuning.Then turn to the home view and click anti-curvature to fully display the tricuspid annulus. Attach to the tricuspid annulus for gating training and utilize the end-breath phase modeling. From the home view, rotate clockwise towards the left atrial anterior wall to reveal the left atrial appendage.Keep turning the catheter clockwise to the left atrium to view the left upper and lower pulmonary veins displayed as a rabbit-ear sign. Rotate to identify the anterior and posterior venous boundaries for left pulmonary vein imaging. Continue turning clockwise to the left atrium, establishing the posterior wall during which the esophagus appears as the double track sign.Keep rotating to observe the right lower pulmonary vein displayed as a three-word sign. Turn in either direction to precisely capture the right pulmonary veins by identifying the anterior and posterior boundaries. Click on palintrope along the long axis to align the catheter tip with the coronary sinus mouth, enhancing the left atrium model.Adjust left bend or right bend to visualize the anterior wall of the left atrium in the long-axis view. Identify and mark the critical anatomical landmarks such as pulmonary vein ostia, left atrial appendage, and other vital sites. The modeling observer scores were 3.40 plus or minus 0.81 in the ICE and 3.02 plus or minus 0.72 in the FAM groups.The observer scores for selecting transseptal puncture sites were 4.62 plus or minus 0.73 in the ICE and 4.29 plus or minus 0.97 in the FAM groups. The pulmonary vein antrum area acquired using the ICE and FAM-based methods correlates with the area acquired by left atrial CT.However, the 95%confidence interval bias obtained using Bland-Altman analysis was narrower in ICE-acquired models than in the FAM-acquired models.