The common marmoset poses unique challenges for neurophysiology due to its small size and lack of gyre as anatomical landmarks. A slight shift of the electrode by just a millimeter can result in significant changes in the retinotopy map. The proposed micro drive system utilizes an XY electrode stage that allows for vertical and horizontal movement on a sub millimeter scale.
Due to the relative novelty of the marmoset monkey as a model for visual neuron sign, awake behaving electrophysiology techniques are still evolving. Current preparations often use semi chronic probes that do not allow access for the positioning mechanisms. This protocol demonstrates a lightweight micro drive for useful linear array recordings, which allow flexible positioning across sessions to map out retinotopy within the chamber.
Long-term cortical damage can be avoided in the preparation when used correctly with the secure drive, slow penetration of tissue, and avoidance of major blood vessels. Additionally, the use of silastic in the craniotomies to avoid infections has been optimized in this technique.
