2D-HPLC-MS Technology Combined with Molecular Network for the Identification of Components in Tibetan Medicine Aconitum pendulum

380 Views

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07:50 min

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December 8th, 2023

DOI :

10.3791/66239-v

December 8th, 2023

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Jing Ma*¹, Hanyu Lu*¹, Jia Liu¹, Ting Wang¹, Xing Fu², Xinmei Xu², Yi Zhang¹, Jing Zhang¹, Xiaolong Xie¹, Yingzhuang Chen³^,⁴, Jinsong Su¹

¹Ethnic Medicine Academic Heritage Innovation Research Center, Meishan Hospital, Chengdu University of Traditional Chinese Medicine, ²State Key Laboratory of Southwestern Chinese Medicine Resources, Innovative Institute of Chinese Medicine and Pharmacy, Chengdu University of Traditional Chinese Medicine, ³Key Laboratory of Chemical Biology & Traditional Chinese Medicine Research, Ministry of Education, ⁴Key Laboratory of Phytochemical R&D of Hunan Province, Hunan Normal University

Summary

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Phytochemical Profile

Herbal Medicines

Chemical Constituents

Complex Mixtures

Therapeutic Properties

Natural Product Discovery

Chapters in this video

0:00

Introduction

1:40

Sample Preparation for 2D‐HPLC‐MS of Aconitum pendulum

3:21

Implementing 2D‐Liquid Chromatography on Aconitum pendulum Samples for Enhanced Compound Separation

4:26

Mass Spectrometry Following the 2D‐LC Operation on Aconitum pendulum Samples

5:47

Integration of Molecular Network Operation to 2D‐HPLC‐MS for the Analysis of Aconitum pendulum Samples

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