JoVE Journal

Biochemistry

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Characterizing Mediated Extracellular Electron Transfer in Lactic Acid Bacteria with a Three-Electrode, Two-Chamber Bioelectrochemical System

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10:23 min

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August 23rd, 2024

DOI :

10.3791/67204-v

August 23rd, 2024

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Robyn A.C. Alba¹, Siliang Li¹, Biki B. Kundu², Caroline M. Ajo-Franklin¹^,²^,³^,⁴^,⁵, Rong Cai¹

¹Department of BioSciences, Rice University, ²PhD Program in Systems, Synthetic, and Physical Biology, Rice University, ³Department of Bioengineering, Rice University, ⁴Department of Chemical and Biomolecular Engineering, Rice University, ⁵Rice Synthetic Biology Institute, Rice University

Summary

Here we present a protocol for characterizing mediated extracellular electron transfer (EET) in lactic acid bacteria using a three-electrode, two-chamber bioelectrochemical system. We illustrate this method with Lactiplantibacillus plantarum and the redox mediator 1,4-dihydroxy-2-naphthoic acid and provide a thorough description of the electrochemical techniques used to evaluate mediated EET.

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Chapters in this video

0:00

Introduction

2:14

Assembly of Bioelectrochemical System Reactors and Initial Culturing of Lactiplantibacillus plantarum for Extracellular Electron Transfer Study

4:11

Setup of Bioelectrochemical System Reactors for Electrochemical Measurements and Culturing of Lactiplantibacillus plantarum