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Generating iPS Cells from MEFS through Forced Expression of Sox-2, Oct-4, c-Myc, and Klf4

36.2K Views

13:02 min

April 7th, 2008

DOI :

10.3791/734-v

April 7th, 2008


Transcript

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IPS Cells

Chapters in this video

0:10

Cellular Reprogramming

3:30

Introduction

4:32

Transfecting HEK293T Cells and Purifying Lentivirus

6:08

Infection of MEFs with Lentivirus

8:56

Picking up the iPS Colonies

11:15

Expansion of iPS Cells

12:32

Conclusion

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