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35.9K Views
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13:02 min
April 7th, 2008
DOI :
10.3791/734-v
Chapters
0:10
Cellular Reprogramming
3:30
Introduction
4:32
Transfecting HEK293T Cells and Purifying Lentivirus
6:08
Infection of MEFs with Lentivirus
8:56
Picking up the iPS Colonies
11:15
Expansion of iPS Cells
12:32
Conclusion
Summary
This video shows the procedure for generating induced pluripotent stem cells using inducible lentivirus that express Oct4, Sox2, c-Myc and Klf4.
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