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Studying Membrane Biogenesis with a Luciferase-Based Reporter Gene Assay

12.8K Views

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13:48 min

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September 7th, 2008

DOI :

10.3791/920-v

September 7th, 2008

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1Department of Molecular and Cell Biology, Harvard, 2Molecular and Metabolic Signalling Centre, Division of Basic Medical Sciences, St. George's University of London

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Membrane Biogenesis
Luciferase based Reporter Gene Assay
Lipid Synthesis Pathways
Experimental System
Phagocytosis
Latex Beads
Membrane Lipids
Gene Expression
Dual Glo Luciferase Assay System

Chapters in this video

0:00

Title

0:49

Introduction

1:21

Preparing Transfections

5:35

Inducing Membrane Biosynthesis via Phagocytosis of Latex Beads

8:07

Performing the Dual-Glo Luciferase Assays

11:28

Data Analysis

13:00

Conclusion

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