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Biochemistry

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Fluorescent Lateral Flow Immunoassay Based on Quantum Dots Nanobeads

1.0K Views

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07:13 min

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June 28th, 2024

DOI :

10.3791/67000-v

June 28th, 2024

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Lingzhi Fan*¹, Yue Luo*², Wannian Yan¹, Huanxing Han³, Pengfei Zhang¹

¹Department of Central Laboratory, Shanghai Skin Disease Hospital, School of Medicine, Tongji University, ²Department of Dermatology, Shanghai Skin Disease Hospital, Institute of Dermatology, School of Medicine, Tongji University, ³Department of Pharmacy, Changzheng Hospital, Naval Medical University

Summary

Here, we describe a protocol for the preparation of quantum dot nanobeads (QDNB) and the detection of disease biomarkers using QDNB-based lateral flow immunoassay strips. The test results can be qualitatively assessed under UV light illumination and quantitatively measured using a fluorescent strip reader within 15 min.

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Chapters in this video

0:00

Introduction

1:54

Preparation of Quantum Dots (QDs) Nanobeads for QDNB‐Based Lateral Flow Immunoassay

3:02

QDNB‐Antibody Conjugates Preparation Using One‐Step Emulsion Evaporation Method

4:46

Lateral Flow Immunoassay Strips Preparation, Assay Operation and its Quantitative Evaluation

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