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Optical Tweezers to Study RNA-Protein Interactions in Translation Regulation

DOI :

10.3791/62589-v

February 12th, 2022

February 12th, 2022

4,168 Views

1Helmholtz Institute for RNA-based Infection Research (HIRI), Helmholtz Zentrum für Infektionsforschung (Helmholtz Centre for Infection Research), 2Medical Faculty, Julius-Maximilians University Würzburg

This protocol presents a complete experimental workflow for studying RNA-protein interactions using optical tweezers. Several possible experimental setups are outlined including the combination of optical tweezers with confocal microscopy.

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