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Transcriptional Analysis by Nascent RNA FISH of In Vivo Trophoblast Giant Cells or In Vitro Short-term Cultures of Ectoplacental Cone Explants

7.8K Views

08:26 min

August 31st, 2016

DOI :

10.3791/54386-v

August 31st, 2016


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Keywords Transcriptional Analysis

Chapters in this video

0:05

Title

0:58

Preparing Secondary Trophoblast Giant Cells (TGCs)

2:11

Fixation and Permeabilization

2:50

RNA FISH: Part 1 (Probe Hybridization)

4:29

RNA FISH: Part 2 (Post-hybridization Washes)

5:43

Results: Probed TGCs

7:15

Conclusion

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