Combining Non-reducing SDS-PAGE Analysis and Chemical Crosslinking to Detect Multimeric Complexes Stabilized by Disulfide Linkages in Mammalian Cells in Culture
9.9K Views
•
09:37 min
•
May 2nd, 2019
DOI :
May 2nd, 2019
•Chapters in this video
0:04
Title
0:34
Harvesting Cells After Blocking Free Cysteine Residues Using Iodoacetamide
2:02
Extraction of Protein and Sample Preparation
3:13
In Vivo Formaldehyde Cross-linking of Endogenous Proteins in U-2 OS Cells
4:30
Fractionation of Nuclei
6:11
Extraction of Protein and Sample Preparation
6:51
SDS-PAGE Analysis
7:53
Results: Analyzing Multimeric Complexes Using a Two-step Method of Non-reducing SDS-PAGE Analysis and Formaldehyde Cross-linking
9:04
Conclusion
Related Videos
Analysis of Zebrafish Larvae Skeletal Muscle Integrity with Evans Blue Dye
9.0K Views
Spatial and Temporal Analysis of Active ERK in the C. elegans Germline
10.1K Views
Directed Differentiation of Primitive and Definitive Hematopoietic Progenitors from Human Pluripotent Stem Cells
10.8K Views
A Standardized Approach for Multispecies Purification of Mammalian Male Germ Cells by Mechanical Tissue Dissociation and Flow Cytometry
11.6K Views
Application of RNAi and Heat-shock-induced Transcription Factor Expression to Reprogram Germ Cells to Neurons in C. elegans
7.5K Views
Cell Aggregation Assays to Evaluate the Binding of the Drosophila Notch with Trans-Ligands and its Inhibition by Cis-Ligands
7.4K Views
Single-cell Photoconversion in Living Intact Zebrafish
5.9K Views
Computational Analysis of the Caenorhabditis elegans Germline to Study the Distribution of Nuclei, Proteins, and the Cytoskeleton
6.2K Views
Combining Histochemical Staining and Image Analysis to Quantify Starch in the Ovary Primordia of Sweet Cherry during Winter Dormancy
6.2K Views
In Vitro Generation of Somite Derivatives from Human Induced Pluripotent Stem Cells
9.2K Views
Copyright © 2025 MyJoVE Corporation. All rights reserved