Controlled Cortical Impact Model of Mouse Brain Injury with Therapeutic Transplantation of Human Induced Pluripotent Stem Cell-Derived Neural Cells

10.7K Views

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09:29 min

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July 10th, 2019

DOI :

10.3791/59561-v

July 10th, 2019

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Orion Furmanski¹^,², Michael D. Nieves¹^,²^,³, Martin L. Doughty¹^,²^,³

¹Center for Neuroscience and Regenerative Medicine, Uniformed Services University, ²Department of Anatomy, Physiology, and Genetics, Uniformed Services University, ³Graduate Program in Neuroscience, Uniformed Services University

Summary

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Controlled Cortical Impact

Mouse Brain Injury

Therapeutic Transplantation

Human Induced Pluripotent Stem Cells

Preclinical Workflow

Traumatic Brain Injury

Cranial Drilling

Unilateral Craniectomy

Stereotaxic Frame

Surgical Drape

Impactor Probe

High speed Rotary Micro Motor Kit

Bone Flap

Drilling Technique

Bleeding Events

Chapters in this video

0:04

Title

0:52

Craniectomy

2:49

Controlled Cortical Impact

3:47

Stereotaxic Transplantation of Cell Suspension

6:38

Sensorimotor Integration Adhesive Tape Removal Test

7:38

Results: Representative Sensorimotor Integration and Human Cell Grafting After Brain Injury

8:32

Conclusion

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