JoVE Journal

Biochemistry

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Rapid Glyco-Qualitative Assessment of Recombinant Proteins Using a Fully Automated System

707 Views

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05:19 min

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June 28th, 2024

DOI :

10.3791/66571-v

June 28th, 2024

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Sayaka Fuseya¹, Ayaka Ono¹, Hiromi Ootani¹, Saho Mizukado¹, Tomomi Obayashi², Nana Tanaka², Hiroko Shimazaki¹^,², Kenji Kajiyama², Moe Ashitomi², Shiori Yasuda², Takenori Miyabe², Kazuhiro Nakamura², Osamu Segawa², Kazumi Sawakami², Atsushi Kuno¹

¹Molecular & Cellular Glycoproteomics Research Group, Cellular and Molecular Biotechnology Research Institute, National Institute of Advanced Industrial Science and Technology, ²Precision System Science Co., Ltd.

Summary

A system for the automated and rapid analysis of glycans on proteins was previously developed. This article presents the protocol for "glyco-qualitative analysis," optimized for a broad spectrum of users, such as those engaged in analyzing glycan structures in biopharmaceuticals and other glycoconjugate materials.

Explore More Videos

Protein Glycosylation

Glycan Structures

Glyco qualification

Biopharmaceuticals

Lectin based Technologies

Glycan Analysis

Glyco biomarkers

Diagnostic Tools

Lectin Microarray

Mass Spectrometry

Automated System

Quantitative Measurement

Recombinant Proteins

Chapters in this video

0:00

Introduction

2:27

Material Preparation and Automatic Glycan Profiling of Proteins

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