Printed Glycan Array: A Sensitive Technique for the Analysis of the Repertoire of Circulating Anti-carbohydrate Antibodies in Small Animals

6.8K Views

•

08:49 min

•

February 14th, 2019

DOI :

10.3791/57662-v

February 14th, 2019

•

Sara Olivera-Ardid*¹, Nailya Khasbiullina*²^,³, Alexey Nokel³, Andrey Formanovsky², Inna Popova², Tatiana Tyrtysh², Roman Kunetskiy², Nadezhda Shilova², Nicolai Bovin²^,⁴, Daniel Bello-Gil¹, Rafael Mañez¹^,⁵

¹Infectious Pathology and Transplantation Division, Bellvitge Biomedical Research Institute (IDIBELL), ²Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry (IBCh), Russian Academy of Sciences, ³Semiotik LLC, ⁴Auckland University of Technology, ⁵Intensive Care Department, Bellvitge University Hospital

Summary

Explore More Videos

Keywords Glycan Array

Anti carbohydrate Antibodies

N hydroxy 6 Cinnamic Derivatized Glass Slides

Blocking Buffer

Buffer

Mice Serum

Immunoglobulin Aggregation

Centrifugation

Incubation Chamber

Chapters in this video

0:04

Title

0:41

Glycochips Production

1:39

Glycan Array Technique

4:44

Analysis of Glycan Array

6:09

Results: Analysis of the Repertoire of Circulating Anti-carbohydrate Antibodies in BALB/c Mice

7:26

Conclusion

Related Videos

13:12

Quantitative Analyses of all Influenza Type A Viral Hemagglutinins and Neuraminidases using Universal Antibodies in Simple Slot Blot Assays

18.2K Views

13:04

An Introduction to Parasitic Wasps of Drosophila and the Antiparasite Immune Response

18.5K Views

09:52

Genetic Manipulation in Δku80 Strains for Functional Genomic Analysis of Toxoplasma gondii

17.0K Views

11:49

Analysis of the Epithelial Damage Produced by Entamoeba histolytica Infection

18.6K Views

10:39

In Vitro Analysis of Myd88-mediated Cellular Immune Response to West Nile Virus Mutant Strain Infection

8.1K Views

10:22

Methods to Investigate the Regulatory Role of Small RNAs and Ribosomal Occupancy of Plasmodium falciparum

8.8K Views

10:32

A Miniaturized Glycan Microarray Assay for Assessing Avidity and Specificity of Influenza A Virus Hemagglutinins

7.9K Views

07:25

In Vitro Methods for Comparing Target Binding and CDC Induction Between Therapeutic Antibodies: Applications in Biosimilarity Analysis

17.6K Views

07:55

Generation of Escape Variants of Neutralizing Influenza Virus Monoclonal Antibodies

11.6K Views

13:14

Generation of Discriminative Human Monoclonal Antibodies from Rare Antigen-specific B Cells Circulating in Blood

10.3K Views