Rapid Antibody Glycoengineering in Chinese Hamster Ovary Cells
3.1K Views
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06:53 min
•
June 2nd, 2022
DOI :
June 2nd, 2022
•Masue Marbiah*1,2, Pavlos Kotidis*1,3, Roberto Donini1,4, Itzcóatl A. Gómez5, Ioscani Jimenez del Val5, Stuart M. Haslam4, Karen M. Polizzi1,2, Cleo Kontoravdi1
1Department of Chemical Engineering, Imperial College London, 2Imperial College Centre for Synthetic Biology, Imperial College London, 3BioPharm Process Research, GlaxoSmithKline Research and Development, 4Department of Life Science, Imperial College London, 5School of Chemical & Bioprocess Engineering, University College Dublin
Chapters in this video
0:04
Introduction
1:00
DsiRNA Transfection
2:17
Buffer Exchange and Sample Concentration
3:19
Glycan Analysis
5:18
Results: Confirmation of RNA Interference and Effect of Fut8 Knockdown
6:12
Conclusion
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