Rapid Antibody Glycoengineering in Chinese Hamster Ovary Cells

3.0K Views

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06:53 min

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June 2nd, 2022

DOI :

10.3791/63872-v

June 2nd, 2022

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Masue Marbiah*¹^,², Pavlos Kotidis*¹^,³, Roberto Donini¹^,⁴, Itzcóatl A. Gómez⁵, Ioscani Jimenez del Val⁵, Stuart M. Haslam⁴, Karen M. Polizzi¹^,², Cleo Kontoravdi¹

¹Department of Chemical Engineering, Imperial College London, ²Imperial College Centre for Synthetic Biology, Imperial College London, ³BioPharm Process Research, GlaxoSmithKline Research and Development, ⁴Department of Life Science, Imperial College London, ⁵School of Chemical & Bioprocess Engineering, University College Dublin

Summary

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Antibody Glycoengineering

Chinese Hamster Ovary Cells

Glycosylation Enzymes

Glyco profile

Biopharmaceutical Companies

Pharmacological Research

Biomedical Research

Mammalian Cell Transfection

Experimental Techniques

Chapters in this video

0:04

Introduction

1:00

DsiRNA Transfection

2:17

Buffer Exchange and Sample Concentration

3:19

Glycan Analysis

5:18

Results: Confirmation of RNA Interference and Effect of Fut8 Knockdown

6:12

Conclusion

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