JoVE Journal
Biochemistry
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Split-Luciferase Reassembly Assay to Measure Endoplasmic Reticulum-Mitochondria Contacts in Live Cells
1.4K Views
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09:09 min
•
October 11th, 2024
DOI :
October 11th, 2024
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Chapters in this video
0:00
Introduction
1:55
Maintaining and Seeding HEK293T Cells for Split‐Rluc Plasmid Transfection
3:38
Polyethyleneimine (PEI)‐Mediated HEK293T Cell Transfection and Post‐Transfection Cell Seeding
5:55
Chemical Treatment and Luciferase Assay to Measure the Level of ER‐Mitochondria Contacts in Live Cells
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ISSN 2578-2037
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