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Optimization of Processing Technology for Tiebangchui with Zanba Based on CRITIC Combined with Box-Behnken Response Surface Method
In This Article
Summary
The present protocol describes an efficient and standard detoxification processing method for Zanba-stir-fried Tiebangchui using CRITIC combined with the Box-Behnken response surface method.
Abstract
The dried root of Aconitum pendulum Busch., called Tiebangchui (TBC) in Chinese, is one of the most famous Tibetan medicines. It is a widely used herb in northwest China. However, many cases of poisoning have occurred because of TBC's intense toxicity and because its therapeutic and toxic doses are similar. Therefore, finding a safe and effective method to reduce its toxicity is an urgent task. A search through the Tibetan medicine classics shows that the processing method of TBC stir-fried with Zanba was recorded in the "Processing specification of Tibetan medicine of Qinghai Province (2010)". However, the specific processing parameters are not yet clear. Thus, this study aims to optimize and standardize the processing technology of Zanba-stir-fried TBC.
First, a single-factor experiment was conducted on four factors: the slice thickness of TBC, amount of Zanba, processing temperature, and time. With monoester and diester alkaloid contents in Zanba-stir-fried TBC as indexes, CRITIC combined with the Box-Behnken response surface method was used to optimize the processing technology of Zanba-stir-fried TBC. The optimized processing conditions of Zanba-stir-fried TBC were a TBC slice thickness of 2 cm, three times more Zanba than TBC, a processing temperature of 125 °C, and 60 min of stir-frying. This study determined the optimized and standard processing conditions for the usage of Zanba-stir-fried TBC, thus providing an experimental basis for the safe clinical use and industrial production of Zanba-stir-fried TBC.
Introduction
The dried root of Aconitum pendulum Busch and A. flavum Hand.-Mazz., one of the most famous Tibetan medicines, is called Tiebangchui (TBC) in Chinese1,2. The dried roots of TBC are helpful in dispelling cold and wind, reducing pain, and calming shock. It was recorded in the first volume of "Drug Standards (Tibetan Medicine) of the Ministry of Health of the People's Republic of China," which states that the dried roots of TBC are commonly used to treat rheumatoid arthritis, bruises, and other cold diseases3. However, the clinical therapeutic dose of TBC is similar to it....
Protocol
The Zanba-stir-fried TBC processing method was optimized and standardized by CRITIC combined with the Box-Behnken response surface method. Benzoylaconitine, aconitine, and 3-deoxyaconitine were used as evaluation indexes during this procedure.
1. Sample solution preparation
- Prepare the reference substance stock solution. Weigh precisely 9.94 mg of benzoylaconitine, 8.49 mg of aconitine, and 6.25 mg of 3-deoxyaconitine (Table of Materials) on an ele.......
Representative Results
In this study, the elution gradient used had a good resolution (Figure 1) for the three index components in Zanba-stir-fried TBC, as determined after repeated debugging. The three index components in Zanba-stir-fried TBC had a good linear relationship within a specific concentration range (Table 2). The precision (Table 5), stability (Table 6), repeatability (Table 7), and sample recovery (Table 8) of Zanba-.......
Discussion
TBC is an important Tibetan medicine with the effects of dispelling cold and relieving pain. It has been mostly used to treat traumatic injury and rheumatic arthralgia in China for thousands of years24,25,26. Diterpenoid alkaloids are both active and toxic ingredients of TBC27,28,29. The main toxic effects of the aconitum alkaloids of T.......
Acknowledgements
This work was financially supported by the National Natural Science Foundation of China (No. 82130113), the China Postdoctoral Science Foundation (No. 2021MD703800), the Science Foundation for Youths of Science & Technology Department of Sichuan Province (No. 2022NSFSC1449), and the "Xinglin Scholars" Research Promotion Program of Chengdu University of Traditional Chinese Medicine (No. BSH2021009).
....Materials
| Name | Company | Catalog Number | Comments |
| 3-Deoxyaconitine | Chengdu Desite Biotechnology Co., Ltd. | DST221109-033 | |
| Aconitine | Chengdu Desite Biotechnology Co., Ltd. | DSTDW000602 | |
| Ammonium acetate | Tianjin Kermel Chemical Reagent Co., Ltd | Chromatographic grade | |
| Benzoylaconitine | Chengdu Desite Biotechnology Co., Ltd. | DSTDB005502 | |
| Design-Expert software | Stat-Ease, Inc., Minneapolis, MN, USA | version 13.0 | |
| Electronic analytical balance | Shanghai Liangping Instruments Co., Ltd. | FA1004 | |
| High performance liquid chromatography | SHIMADZU Co., Ltd. | LC-20A | |
| High-speed smashing machine | Beijing Zhongxing Weiye Instrument Co., Ltd. | FW-100 | |
| Millipore filter | Tianjin Jinteng Experimental Equipment Co., Ltd | φ13 0.22 Nylon66 | |
| stir-Fry machine | Changzhou Maisi Machinery Co., Ltd | Type 5 | |
| Tiebangchui | Gannan Baicao Biotechnology Development Co., Ltd | 20211012 | |
| Ultra pure water systemic | RephiLe Bioscience, Ltd. | Genie G | |
| Ultrasonic cleansing machine | Ningbo Xinyi Ultrasonic Equipment Co., Ltd | SB2200 | |
| Zanba | 27 Chuanzang Road, Ganzi County | - |
References
- Li, C. Y., et al. Aconitum pendulum and Aconitum flavum: A narrative review on traditional uses, phytochemistry, bioactivities and processing methods. Journal of Ethnopharmacology. 292, 115216 (2022).
- Wang, J., Meng, X. H., Chai, T., Yang, J. L., Shi, Y. P.
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