Prediction of Cdk1-specific Phosphorylation Sites and Expression of CENP-F in Escherichia coli
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Recombitant Protein Purification by Glutathione-affinity Chromatography and Gel Filtration Followed by In Vitro Kinase Assay with Cdk1/Cyclin B
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Results: Use of In Vitro Kinase Assay to Study Cdk1-specific Phosphorylation Sites in CENP-F
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Conclusion
副本
The overall goal of this in vitro kinase assay is to identify phosphorylation sites in a protein of interest that are specific for the kinase cyclin-dependent kinase 1. The identification of CDK1 specific phosphorylation sites is important as they
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Cyclin-dependent kinase 1 (Cdk1) is activated in the G2 phase of the cell cycle and regulates many cellular pathways. Here, we present a protocol for an in vitro kinase assay with Cdk1, which allows the identification of Cdk1-specific phosphorylation sites for establishing cellular targets of this important kinase.