JoVE Logo
教师资源中心

登录

Identification of Novel CK2 Kinase Substrates Using a Versatile Biochemical Approach

DOI :

10.3791/59037-v

February 21st, 2019

February 21st, 2019

7,086 Views

1Department of Biochemistry and Molecular Biology, Drexel University College of Medicine

The objective of this protocol is to label, enrich, and identify substrates of protein kinase CK2 from a complex biological sample such as a cell lysate or tissue homogenate. This method leverages unique aspects of CK2 biology for this purpose.

Tags

Keywords CK2

-- Views

Related Videos

article

A Protein Preparation Method for the High-throughput Identification of Proteins Interacting with a Nuclear Cofactor Using LC-MS/MS Analysis

article

Quantification of Bacterial Histidine Kinase Autophosphorylation Using a Nitrocellulose Binding Assay

article

Assaying Protein Kinase Activity with Radiolabeled ATP

article

Biochemical and Structural Characterization of the Carbohydrate Transport Substrate-binding-protein SP0092

article

Identification of Cyclin-dependent Kinase 1 Specific Phosphorylation Sites by an In Vitro Kinase Assay

article

The Identification of Sea Lamprey Pheromones Using Bioassay-Guided Fractionation

article

Enhancing the Engraftment of Human Induced Pluripotent Stem Cell-derived Cardiomyocytes via a Transient Inhibition of Rho Kinase Activity

article

Characterization at the Molecular Level using Robust Biochemical Approaches of a New Kinase Protein

article

Kinase Inhibitor Screening In Self-assembled Human Protein Microarrays

article

Nonradioactive Assay to Measure Polynucleotide Phosphorylation of Small Nucleotide Substrates

JoVE Logo

政策

使用条款

隐私

科研

教育

关于 JoVE

版权所属 © 2024 MyJoVE 公司版权所有,本公司不涉及任何医疗业务和医疗服务。