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JoVE Journal

Environment

This content is Open Access.

Characterizing Microbiome Dynamics – Flow Cytometry Based Workflows from Pure Cultures to Natural Communities

11.6K Views

09:57 min

July 12th, 2018

July 12th, 2018

11,595 Views

0:04

Title

0:50

Biogas Community Drying and Activated Sludge Community (ASC) Cell Stabilization

2:29

ASC Staining

3:46

Bead Calibration and ASC Sample Analysis

5:29

Cytometric Barcode

7:21

Results: Representative Microbial Evolution Analyses

9:08

Conclusion

副本

Flow cytometric analysis has proven valuable for investigating pure cultures and monitoring microbial community dynamics. We present three comprehensive workflows, from sampling to data analysis, for pure cultures and complex communities in clear medium as well as in challenging matrices, respectively.

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