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JoVE Journal

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Characterizing Microbiome Dynamics – Flow Cytometry Based Workflows from Pure Cultures to Natural Communities

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09:57 min

July 12th, 2018

July 12th, 2018

11,624 Views

0:04

Title

0:50

Biogas Community Drying and Activated Sludge Community (ASC) Cell Stabilization

2:29

ASC Staining

3:46

Bead Calibration and ASC Sample Analysis

5:29

Cytometric Barcode

7:21

Results: Representative Microbial Evolution Analyses

9:08

Conclusion

Transkript

Flow cytometric analysis has proven valuable for investigating pure cultures and monitoring microbial community dynamics. We present three comprehensive workflows, from sampling to data analysis, for pure cultures and complex communities in clear medium as well as in challenging matrices, respectively.

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