Cell Culture, and the Collection of EVs and RNA-associated Biomolecules
2:54
RNA Extraction and Quality Control
4:20
Library Construction and Quality Control
9:04
Results: Collecting exRNA from hMSC for Next Generation Sequencing
10:31
Conclusion
Transcript
This is the first paper that addresses technical problems in sequencing small RNAs derived from extracellular vesicles from cells. NGS samples require stringent preparation and quality control. Due to the nature of EVs, the QC process of EV sample
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This protocol demonstrates how to purify extracellular microRNAs from cell culture media for small RNA library construction and next generation sequencing. Various quality control checkpoints are described to allow readers to understand what to expect when working with low input samples like exRNAs.
