This protocol is significant as it allows for elucidation of the role cellular forces play in many physiological and pathological processes, such as wound healing and cancer metastases, for example. The main advantage of this mechanics-based techn
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Here, we present a mechanics-based protocol to disrupt the gap junction connexin 43 and measure the subsequent impact this has on endothelial biomechanics via observation of tractions and intercellular stresses.