Murine Oropharyngeal Instillation of Apoptotic Cells
4:45
Bronchoalveolar Lavage Fluid Collection and Processing
6:30
Calculation of Alveolar Macrophage Efferocytic Index
7:06
Results: O3 Exposure Induces Pulmonary Inflammation and Injury
8:14
Conclusion
副本
The protocol described here measures lung macrophage efferocytosis after exposure to a criteria air pollutant ozone. These data indicate that ozone exposure decreases alveolar macrophage efferocytosis and thus could be a mechanism for why elevated
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This manuscript describes a protocol for determining whether exposure to ozone, a criteria air pollutant, impairs alveolar macrophage efferocytosis in vivo. This protocol utilizes commonly used reagents and techniques and can be adapted to multiple models of pulmonary injury to determine effects on alveolar macrophage efferocytosis.
