Results: Representative Mouse Alveolar Macrophage Phagocytosis and Bacteria Clearance
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Conclusion
副本
Pseudomonas aeruginosa is a BSL-2 level pathogen. Remember to follow all BSL-2 level safety practices when handling this organism. Here we will demonstrate primary cell isolation, in vitro phagocytosis, and phagocytic pathway analysis, and in vivo
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Here we report common methods to analyze the phagocytic function of murine alveolar macrophages and bacterial clearance from the lung. These methods study in vitro phagocytosis of fluorescein isothiocyanate beads and in vivo phagocytosis of Pseudomonas aeruginosa Green Fluorescent Protein. We also describe a method for clearing P. aeruginosa in mice.
