Determination of Bacterial Concentration Using the Drop Plate Method
4:55
RT-qPCR Detection of Bacterial 16S rRNA
7:00
Results: CSE-Treatment Increases Bacterial Load
8:23
Conclusion
副本
This protocol uses three approaches to determine if cigarette smoke affects pseudomonas bacterial load in lung epithelial cells. This method can be expanded to endothelial cells or other cell taps. Preparation of the cigarette smoke extraction, ba
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Described here is a protocol to study how cigarette smoke extract affects bacterial colonization in lung epithelial cells.
