Results: Validation of Hoechst/PI Cytotoxicity Assay in Leukemia Cells
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Conclusion
Transcript
This method will allow researchers to assess cellular viability independently of mitochondrial function. And it is amenable to high throughput drug screening efforts. This technique is fast, accurate, inexpensive, and allows the determination of c
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The protocol describes a rapid, high-throughput, reliable, inexpensive, and unbiased assay for efficiently determining cellular viability. This assay is particularly useful when cells' mitochondria have been damaged, which interferes with other assays. The assay uses automated counting of cells stained with two nuclear dyes – Hoechst 33342 and propidium iodide.
