Embryo Microinjection and Knockout Mutant Identification of CRISPR/Cas9 Genome-Edited Helicoverpa Armigera (Hübner)

0:29

Design of Gene-Specific Primers and Preparation of sgRNA and Embryos

2:18

Microinjection of Embryos

3:47

Post-Injection Insect Rearing

4:41

Knock-Out Mutation Detection

5:27

Results: Selection and Synthesis of Target sgRNAs and Detection of Mutants by PCR and Gel Electrophoresis

6:08

Conclusion