Multiple Fluorescent Staining with Neurofilament 200 (NF200), Vesicular Acetylcholine Transporter (VAChT), Alpha‐Bungarotoxin (α‐BTX), and Phalloidin (Pha)
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Observation and Analyses
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Results: Investigating Spatial Correlation of Neuromuscular Junction in Rat Medial Gastrocnemius Muscle
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Conclusion
副本
This protocol can be used to evaluate integrity and plasticity of a neuromuscular junction under normal and pathological conditions. This technique is a to simultaneously label the Schwann cells and pre and post lab animals on single muscle sectio
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The protocol shows a method to examine spatial correlation among the pre-synaptic terminals, post-synaptic receptors, and peri-synaptic Schwann cells in the rat medial gastrocnemius muscle using fluorescent immunohistochemistry with different biomarkers, namely, neurofilament 200, vesicular acetylcholine transporter, alpha-bungarotoxin, and S100.