Setting up the Protocol with the Microplate Reader, Establishing the Elicitation System, and Measuring Real-Time ROS Production
3:42
Results: ROS Induction, the Total Amount of ROS Generated, and ROS Production in the Exposed Cells of the Cut Edge
5:07
Conclusion
副本
This protocol describes an optimized L-012-based chemiluminescence method to detect ROS production in real time upon PAMP elicitation in rice tissues. This method is easy, standardized, and highly reproducible under firmly controlled conditions. D
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Here, we describe a method for the real-time detection of apoplastic reactive oxygen species (ROS) production in rice tissues in pathogen-associated molecular pattern-triggered immune response. This method is simple, standardized, and generates highly reproducible results under controlled conditions.