An Efficient and Fast Method for Labeling and Analyzing Mouse Glomeruli

1.2K Views

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09:50 min

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February 9th, 2024

DOI :

10.3791/65973-v

February 9th, 2024

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Lin Bai¹^,², Yaping Wu², Wenshu Dai¹, Qiuxiao Shi², Lei Wu², Jie Zhang¹^,², Lily Zheng³

¹Key Laboratory of Transplant Engineering and Immunology, NHFPC, West China Hospital, Sichuan University, ²Core Facility of West China Hospital, Sichuan University, ³Regenerative Medicine Research Center, West China Hospital, Sichuan University

Transcript

Summary

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CUBIC Tissue Clearing

Light sheet Microscopy

Renal Function

Biological Imaging

Glomeruli Counting

FITC Dextran Injection

Three dimensional Imaging

Renal Research

Lab Techniques

Biotargets

Kidney Disease Research

Chapters in this video

0:00

Introduction

1:33

Tissue Clearing of Mouse Kidneys for Confocal Microscopy

2:54

Confocal and Light Sheet Fluorescence Microscopy (LSFM) Imaging of Cleared Mouse Kidneys

7:00

Data Processing and Quantification of Glomeruli from Microscopy Images

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