High-Quality Seizure-Like Activity from Acute Brain Slices Using a Complementary Metal-Oxide-Semiconductor High-Density Microelectrode Array System

1.9K Views

•

06:28 min

•

September 27th, 2024

DOI :

10.3791/67065-v

September 27th, 2024

•

Melissa L. Blotter*¹^,², Isaac W. Stubbs*¹^,², Jacob H. Norby*¹^,², Maxwell Holmes¹^,², Ben Kearsley³, Alexis Given¹, Kutter Hine¹^,⁴, Micah R. Shepherd⁵, R. Ryley Parrish¹^,²

¹Department of Cell Biology and Physiology, Brigham Young University, ²Neuroscience Center, Brigham Young University, ³Department of Statistics, Brigham Young University, ⁴Department of Biology, Brigham Young University, ⁵Department of Physics and Astronomy, Brigham Young University

Summary

Explore More Videos

Seizure like Activity

Acute Brain Slices

CMOS HD MEA System

High density Microelectrode Array

Electrophysiological Data

Status Epilepticus

Local Field Potentials

Neurophysiological Activity

Neuronal Unit Activity

Experimental Protocol

Treatment Methods

Brain Slice Recording

Electrical Noise

Chapters in this video

0:00

Introduction

1:00

Equipment Preparation for CMOS‐HD‐MEA Experimentation

2:23

Chip Preparation and Placement of Brain Slice to Record High‐Quality Seizure‐Like Activity Using CMOS‐HD‐MEA System

Related Videos

09:06

A Low-cost Method for Analyzing Seizure-like Activity and Movement in Drosophila

13.5K Views

10:24

Electrophysiological and Morphological Characterization of Neuronal Microcircuits in Acute Brain Slices Using Paired Patch-Clamp Recordings

17.1K Views

09:39

Direct-current Stimulation and Multi-electrode Array Recording of Seizure-like Activity in Mice Brain Slice Preparation

10.4K Views

10:53

Preparation of Acute Brain Slices Using an Optimized N-Methyl-D-glucamine Protective Recovery Method

45.6K Views

09:51

Recording Synaptic Plasticity in Acute Hippocampal Slices Maintained in a Small-volume Recycling-, Perfusion-, and Submersion-type Chamber System

11.4K Views

08:23

Adaptation of Microelectrode Array Technology for the Study of Anesthesia-induced Neurotoxicity in the Intact Piglet Brain

9.4K Views

10:24

Recording and Modulation of Epileptiform Activity in Rodent Brain Slices Coupled to Microelectrode Arrays

14.5K Views

07:36

Preparation of a High-quality Primary Cell Culture from Fish Pituitaries

15.1K Views

07:58

Acute Mouse Brain Slicing to Investigate Spontaneous Hippocampal Network Activity

8.8K Views

07:56

A Plate-Based Assay for the Measurement of Endogenous Monoamine Release in Acute Brain Slices

3.2K Views