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Preparation of a High-quality Primary Cell Culture from Fish Pituitaries

DOI :

10.3791/58159-v

August 28th, 2018

August 28th, 2018

14,685 Views

1Department of Basic Sciences and Aquatic Medicine, Faculty of Veterinary Medicine, Norwegian University of Life Sciences, 2Department of Oral Biology, Faculty of Dentistry, University of Oslo

Here we describe a protocol to prepare and maintain primary pituitary cell cultures from medaka (Oryzias latipes). The optimized conditions in this protocol take important parameters such as temperature, osmolality, and pH into consideration by mimicking the physiological conditions of the fish, thereby enabling physiologically more meaningful results.

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Primary Cell Culture

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