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09:10 min
October 18th, 2010
DOI :
10.3791/2308-v
Kapitel
0:00
Title
1:32
Introduction
2:07
Surface Sampling with Sterile Adhesive Tape
3:33
Solid-phase Enrichment
4:54
Fixation and Dehydration
5:35
Hybridization
6:46
Detection
7:43
FISH and FACS Analyses on Tomatoes
8:32
Conclusion
Summary
This protocol describes a simple adhesive-tape-based approach for sampling of tomato and other fresh produce surfaces, followed by rapid whole cell detection of Salmonella using fluorescence in situ hybridization (FISH).
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