Besonderer Dank geht an Chris Kassotis und Audrey Bailey für die kritische Durchsicht des Manuskripts und Dr. Scott Korte, Joseph Beeman, Alison Curfman, Paul Kimball, Bridget Neibreggue, Jacob Redel, Amy Schröder, Maija Steinberg, und Stacey Winkeler für ihre Unterstützung bei der Optimierung dieses Modell in unserem Labor. Die Finanzierung wurde von der Clinical Biodetectives Training Grant (NIH T90) (KEP), University of Missouri Life Sciences Undergraduate Research Opportunities Program, MU Research Council, MU Research Board Zuschüsse und NIH R21HD056441 (SCN) zur Verfügung gestellt.

<ol>
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Keine Interessenskonflikte erklärt.

Es gibt mehrere kritische Parameter, bemerkt während der Durchführung der chirurgischen Induktion von Endometriose bei Mäusen sollte. Erstens ist die Endometriose ein Östrogen abhängig Krankheit und als solche diese Operation sollte in gesunden Tieren oder alternativ in ovarektomierten Tiere mit Östrogenen 20 ergänzt durchgeführt werden. Zweitens muss die Naht Endometriumbiopsien der arteriellen Kaskade mit äußerster Sorgfalt durchgeführt werden. Wir haben festgestellt, dass mit nur zwei relativ lo...

<table border="1"><tbody><tr><td> Name des Reagenzes / Ausrüstung </td><td> Firma </td><td> Katalog-Nummer </td></tr><tr><td> Wax Bleistift </td><td> Fischer </td><td> NC9954135 </td></tr><tr><td> Objektträger aus Glas </td><td> Fischer </td><td> 12-550-433 </td></tr><tr><td> Pipette </td><td> Fischer </td><td> S79383 </td></tr><tr><td> Standard-Lichtmikroskop für die Bewertung Vaginalzytologie Abstrichen </td><td></td><td></td></tr><tr><td> Buprenorphin HCL c3 (CARJET) 10X1ml </td><td> Butler Tiergesundheit Versorgung </td><td> 022891 </td></tr><tr><td> Sterile Phosphat-gepufferter Kochsalzlösung (PBS) </td><td> Gibco </td><td> 14040-117 </td></tr><tr><td> 10.000 U / ml Penicillin, 10.000 pg / ml Streptomycin in 0,85% NaCl </td><td> Hyclone </td><td> SV30010 </td></tr><tr><td> Isofluran </td><td> AbbottTiergesundheit </td><td> 05260-05 </td></tr><tr><td> Isofluran nicht Rückatmungssystemen System </td><td></td><td></td></tr><tr><td> Recirculating Warmwasser Heizkissen </td><td></td><td></td></tr><tr><td> 30 ml Spritze Mantel </td><td> Fischer </td><td> 14 bis 823-16G </td></tr><tr><td> Puderfrei sterile Handschuhe </td><td> Fisherbrand </td><td> 19020558 </td></tr><tr><td> Augensalbe </td><td> Große Pharmaceuticals </td><td> 10033691 </td></tr><tr><td> Kleine elektrische Haarschneidemaschine </td><td> Wahl </td><td> 9861-600 </td></tr><tr><td> Chlorhexidin scrub </td><td> Fischer </td><td> NC9863042 </td></tr><tr><td> 70% Ethanol </td><td></td><td></td></tr><tr><td> Polylined sterilen Bereich </td><td> Busse Krankenhaus Disposables </td><td> 696 </td></tr><tr><td> Größe 3 Skalpell </td><td> Fischer </td><td> 22-079-657 </td></tr><tr><td> Number 10 Skalpellklingen </td><td> Fischer </td><td> 22-079-681 </td></tr><tr><td> Kleine chirurgische Scheren </td><td> Roboz </td><td> RS-5850 </td></tr><tr><td> Kleine gezackte semi-gebogenen Pinzette </td><td> Roboz </td><td> RS-5135 </td></tr><tr><td> 5-0 schwarz geflochten Seidenfaden </td><td> Ethicon </td><td> K870H </td></tr><tr><td> Sterilisiert Pyrexglas Petrischalen </td><td> Corning </td><td> 70160-101 </td></tr><tr><td> 2 mm Biopsiestanze </td><td> Miltex </td><td> 33-31 </td></tr><tr><td> Sterile Gaze </td><td> Kendall </td><td> 1806 </td></tr><tr><td> 6-0 schwarz Monofilament Ethilon Nylonnaht </td><td> Ethicon </td><td> 697G </td></tr><tr><td> Needle-Treiber (optional) </td><td> World Precision Instruments </td><td> 500023 </td></tr><tr><td> 5-0 ungefärbt geflochten beschichtet vicryl Naht </td><td> Ethicon </td><td> J490G </td></tr><tr><td> 9mm Autoclip Wundklammern </td><td> Becton Dickinson </td><td> 427631 </td></tr><tr><td> Autoclip Applier &amp; Entferner </td><td> Becton Dickinson </td><td> 427630 </td></tr><tr><td> 23G Nadel </td><td> Becton Dickinson </td><td> 305193 </td></tr><tr><td> 1cc Spritze </td><td> Becton Dickson </td><td> 301025 </td></tr><tr><td> 5x Lupe Standfuß (optional) </td><td> Fischer </td><td> 14-648-23 </td></tr><tr><td> 10% gepuffertem Formalin </td><td> Fischer </td><td> SF100-4 </td></tr><tr><td> Taster </td><td> Roboz </td><td> RS-6466 </td></tr><tr><td> Verarbeitung / Einbettkassetten </td><td> Fischer </td><td> 15 bis 197-700A </td></tr><tr><td> Biopsie Schaumstoff-Pads </td><td> Fischer </td><td> 22-038-222 </td></tr><tr><td> RNAqueous RNA Isolation Kit </td><td>Ambion </td><td> AM1912 </td></tr><tr><td> Flüssiger Stickstoff </td><td></td><td></td></tr><tr><td> Schnappdeckel Mikrozentrifuge flachen Oberrohr </td><td> Fischer </td><td> 02-681-240 </td></tr><tr><td> Ketamin (optional) </td><td> Simga </td><td> K4138 </td></tr><tr><td> Domitor (Medetomidinhydrochlorid) (optional) </td><td> Tocris </td><td> 2023 </td></tr><tr><td> Antisedan (Atipamezol) (optional) </td><td> Sigma </td><td> A9611 </td></tr></tbody></table>

mouse model of surgically-induced endometriosis by auto-transplantation of uterine tissue

Die Endometriose ist eine chronische, schmerzhafte Erkrankung, deren Ätiologie ist unbekannt. Darüber hinaus kann die Behandlung der Endometriose erfordern laparoskopische Entfernung der Läsionen und / oder chronischen pharmazeutische Behandlung von Schmerzen und Unfruchtbarkeit Symptome. Die Kosten im Zusammenhang mit Endometriose hat bei 22 Milliarden Dollar pro Jahr in den Vereinigten Staaten von Amerika 1 geschätzt. Um unser Verständnis der zugrunde liegenden Mechanismen dieser rätselhaften Krankheit sind Tiermodelle eingesetzt. Primaten entwickeln spontan Endometriose und somit Primatenmodelle am ehesten die Krankheit bei Frauen ähneln. Nager-Modelle, sind aber kostengünstiger und leicht verfügbar 2. Das Modell, das wir hier beschreiben, beinhaltet eine autologe Transfer von Gebärmutter-Gewebe der Darmwand Mesenterium (Abbildung 1) und wurde zum ersten Mal in der Ratte 3 entwickelt und später an der Maus 4 übertragen. Das Ziel der autologen Nagetier Modell der chirurgisch-induzierten Endometriose ist zu imitierendie Krankheit bei Frauen. Wir und andere haben bereits gezeigt, dass die veränderte Genexpressionsmuster in Endometrioseherde von Mäusen oder Ratten Spiegel bei Frauen mit der Krankheit 5,6 beobachtet wurden. Ein Vorteil der Durchführung der Operation in der Maus ist, dass die Fülle von transgenen Mauslinien zur Verfügung können die Forscher bei der Bestimmung der Rolle von spezifischen Komponenten wichtig für die Gründung und das Wachstum der Endometriose helfen. Ein alternatives Modell, in dem ausgeschnittenen menschlichen Endometrium-Fragmente an das Bauchfell von immungeschwächten Mäusen eingebracht werden, ist ebenfalls weit verbreitet, sondern wird durch das Fehlen eines normalen Immunsystems, die Gedanken zu sein bei der Endometriose 2,7 wichtig ist begrenzt. Wichtig ist, dass die Maus-Modell der chirurgisch induzierten Endometriose ein vielseitiges Modell, das verwendet wurde, zu untersuchen, wie das Immunsystem 8, Hormone 9,10 und Umweltfaktoren 11,12 Endometriose sowie die Auswirkungen von Endometriose auf fert beeinflussenility 13 und Schmerzen 14.

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Mouse Model of Surgically-induced Endometriosis by Auto-transplantation of Uterine Tissue

Eine Beschreibung der chirurgischen Induktion von Endometriose bei Mäusen und Ratten durch auto-Transplantation von Gebärmutter-Gewebe auf die arterielle Kaskade der Darm Mesenterium.

Maus-Modell der chirurgisch-induzierten Endometriose durch Auto-Transplantation von Gebärmutter-Gewebe

Endometriosis is a chronic, painful disease whose etiology remains unknown. Furthermore, treatment of endometriosis can require laparoscopic removal of ...

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28.5K Aufrufe.  University of Missouri. Eine Beschreibung der chirurgischen Induktion von Endometriose bei Mäusen und Ratten durch auto-Transplantation von Gebärmutter-Gewebe auf die arterielle Kaskade der Darm Mesenterium.

Serie: Mouse Model of Surgically-induced Endometriosis by Auto-transplantation of Uterine Tissue

Mouse Model of Intraluminal MCAO: Cerebral Infarct Evaluation by Cresyl Violet Staining

Stroke is the third cause of mortality and the leading cause of disability in the World. Ischemic stroke accounts for approximately 80% of all strokes. However, the thrombolytic tissue plasminogen activator (tPA) is the only treatment of acute ischemic stroke that exists. This led researchers to develop several ischemic stroke models in a variety of species. Two major types of rodent models have been developed: models of global cerebral ischemia or focal cerebral ischemia. To mimic ischemic stroke in patients, in whom approximately 80% thrombotic or embolic strokes occur in the territory of the middle cerebral artery (MCA), the intraluminal middle cerebral artery occlusion (MCAO) model is quite relevant for stroke studies. This model was first developed in rats by Koizumi et al. in 1986 1. Because of the ease of genetic manipulation in mice, these models have also been developed in this species 2-3.
	Herein, we present the transient MCA occlusion procedure in C57/Bl6 mice. Previous studies have reported that physical properties of the occluder such as tip diameter, length, shape, and flexibility are critical for the reproducibility of the infarct volume 4. Herein, a commercial silicon coated monofilaments (Doccol Corporation) have been used. Another great advantage is that this monofilament reduces the risk to induce subarachnoid hemorrhages. Using the Zeiss stereo-microscope Stemi 2000, the silicon coated monofilament was introduced into the internal carotid artery (ICA) via a cut in the external carotid artery (ECA) until the monofilament occludes the base of the MCA. Blood flow was restored 1 hour later by removal of the monofilament to mimic the restoration of blood flow after lysis of a thromboembolic clot in humans. The extent of cerebral infarct may be evaluated first by a neurologic score and by the measurement of the infarct volume. Ischemic mice were thus analyzed for their neurologic score at different post-reperfusion times. To evaluate the infarct volume, staining with 2,3,5-triphenyltetrazolium chloride (TTC) was usually performed. Herein, we used cresyl violet staining since it offers the opportunity to test many critical markers by immunohistochemistry. In this video, we report the MCAO procedure; neurological scores and the evaluation of the infarct volume by cresyl violet staining.

The intraluminal middle cerebral occlusion model in mice is herein presented. The extent of cerebral infarct is evaluated by a neurologic score and cresyl violet staining, an alternative staining to TTC, offering the great advantage to test in parallel many interest markers.

Maus-Modell der Intraluminal MCAO: Hirninfarkt Evaluation durch Cresylviolett Staining

Stroke is the third  cause of mortality and the leading cause of disability in the World. Ischemic  stroke accounts for approximately 80% of all strokes. ...

Forschung

Medizin

Implantation of Inferior Vena Cava Interposition Graft in Mouse Model

Biodegradable scaffolds seeded with bone marrow mononuclear cells (BMCs) are often used for reconstructive surgery to treat congenital cardiac anomalies. The long-term clinical results showed excellent patency rates, however, with significant incidence of stenosis. To investigate the cellular and molecular mechanisms of vascular neotissue formation and prevent stenosis development in tissue engineered vascular grafts (TEVGs), we developed a mouse model of the graft with approximately 1 mm internal diameter. First, the TEVGs were assembled from biodegradable tubular scaffolds fabricated from a polyglycolic acid nonwoven felt mesh coated with &#949;-caprolactone and L-lactide copolymer. The scaffolds were then placed in a lyophilizer, vacuumed for 24 hr, and stored in a desiccator until cell seeding. Second, bone marrow was collected from donor mice and mononuclear cells were isolated by density gradient centrifugation. Third, approximately one million cells were seeded on a scaffold and incubated O/N. Finally, the seeded scaffolds were then implanted as infrarenal vena cava interposition grafts in C57BL/6 mice. The implanted grafts demonstrated excellent patency (&#62;90%) without evidence of thromboembolic complications or aneurysmal formation. This murine model will aid us in understanding and quantifying the cellular and molecular mechanisms of neotissue formation in the TEVG.

To improve our knowledge of cellular and molecular neotissue formation, a murine model of the TEVG was recently developed. The grafts were implanted as infrarenal vena cava interposition grafts in C57BL/6 mice. This model achieves similar results to those achieved in our clinical investigation, but over a far shortened time-course.

Die Implantation von Vena Cava Interposition Graft in Maus-Modell

Biodegradable scaffolds seeded with bone marrow mononuclear cells (BMCs) are often used for reconstructive surgery to treat congenital cardiac anomalies. ...

Transplantation of Pulmonary Valve Using a Mouse Model of Heterotopic Heart Transplantation

Tissue engineered heart valves, especially decellularized valves, are starting to gain momentum in clinical use of reconstructive surgery with mixed results. However, the cellular and molecular mechanisms of the neotissue development, valve thickening, and stenosis development are not researched extensively. To answer the above questions, we developed a murine heterotopic heart valve transplantation model. A heart valve was harvested from a valve donor mouse and transplanted to a heart donor mouse. The heart with a new valve was transplanted heterotopically to a recipient mouse. The transplanted heart showed its own heartbeat, independent of the recipient&#8217;s heartbeat. The blood flow was quantified using a high frequency ultrasound system with a pulsed wave Doppler. The flow through the implanted pulmonary valve showed forward flow with minimal regurgitation and the peak flow was close to 100 mm/sec. This murine model of heart valve transplantation is highly versatile, so it can be modified and adapted to provide different hemodynamic environments and/or can be used with various transgenic mice to study neotissue development in a tissue engineered heart valve.

In order to understand the cellular and molecular mechanisms underlying neotissue formation and stenosis development in tissue engineered heart valves, a murine model of heterotopic heart valve transplantation was developed. A pulmonary heart valve was transplanted to recipient using the heterotopic heart transplantation technique.

Transplantation von Pulmonalklappe Mit einem Maus-Modell der heterotopen Herztransplantation

Tissue engineered heart valves, especially decellularized valves, are starting to gain momentum in clinical use of reconstructive surgery with mixed ...

Utero-tubal Embryo Transfer and Vasectomy in the Mouse Model

The transfer of preimplantation embryos to a surrogate female is a required step for the production of genetically modified mice or to study the effects of epigenetic alterations originated during preimplantation development on subsequent fetal development and adult health. The use of an effective and consistent embryo transfer technique is crucial to enhance the generation of genetically modified animals and to determine the effect of different treatments on implantation rates and survival to term. Embryos at the blastocyst stage are usually transferred by uterine transfer, performing a puncture in the uterine wall to introduce the embryo manipulation pipette. The orifice performed in the uterus does not close after the pipette has been withdrawn, and the embryos can outflow to the abdominal cavity due to the positive pressure of the uterus. The puncture can also produce a hemorrhage that impairs implantation, blocks the transfer pipette and may affect embryo development, especially when embryos without zona are transferred. Consequently, this technique often results in very variable and overall low embryo survival rates. Avoiding these negative effects, utero-tubal embryo transfer take advantage of the utero-tubal junction as a natural barrier that impedes embryo outflow and avoid the puncture of the uterine wall. Vasectomized males are required for obtaining pseudopregnant recipients. A technique to perform vasectomy is described as a complement to the utero-tubal embryo transfer.

Utero-tubal embryo transfer uses the utero-tubal junction as a barrier to prevent the embryo outflow that may occur when performing uterine transfer. Vasectomized males are required to obtain pseudopregnant recipients for embryo transfer. Both techniques are discussed.

Utero-Tubenembryotransfer und Vasektomie im Maus-Modell

The transfer of preimplantation embryos to a surrogate female is a required step for the production of genetically modified mice or to study the effects ...

Biologie

A Syngeneic Murine Model of Endometriosis using Naturally Cycling Mice

Endometriosis is a leading cause of pelvic pain and infertility. It is defined by the presence of endometrial tissue in extrauterine locations. The development of novel therapies and diagnostic tools for endometriosis has been limited due in part to challenges in studying the disease. Outside of primates, few mammals menstruate, and none develop spontaneous endometriosis. Rodent models are popular but require artificial induction of endometriosis, with many utilizing either immunocompromised mice or surgically induced disease. Recently, more attention has been given to models involving intraperitoneal injection. We present a murine model of endometriosis that integrates several features of existing endometriosis models into a novel, simplified system that relies on microscopic quantification in lieu of subjective grading. In this model, we perform hormonal stimulation of donor mice, intraperitoneal injection, systematic abdominal survey and tissue harvest, and histologic quantification that can be performed and verified at any time after necropsy. This model requires minimal resources and training; does not require expertise by lab technicians in murine survival surgery or in the identification of gross endometriotic lesions; can be used in immunocompromised, immunocompetent, and/or mutant mice; and reliably creates endometriotic lesions that are histologically consistent with human endometriotic disease.

Many rodent models of endometriosis are limited by technical complexity, reproducibility, and/or need for immunocompromised animals or special reporter mice. We present a simplified system of lesion induction using any experimental mouse with an independently verifiable, objective scoring system and with no requirement for ovariectomy or survival surgery.

Ein syngenes Murinmodell der Endometriose mit natürlich radfahrenden Mäusen

Endometriosis is a leading cause of pelvic pain and infertility. It is defined by the presence of endometrial tissue in extrauterine locations. The ...

Noninvasive Monitoring of Lesion Size in a Heterologous Mouse Model of Endometriosis

Here, we describe a protocol for the implementation of a heterologous mouse model in which progression of endometriosis can be assessed in real time through noninvasive monitoring of fluorescence emitted by implanted ectopic human endometrial tissue. For this purpose, biopsies of human endometrium are obtained from donor women ongoing oocyte donation. Human endometrial fragments are cultured in the presence of adenoviruses engineered to express cDNA for the reporter fluorescent protein mCherry. Upon visualization, labeled tissues with an optimal rate of fluorescence after infection are subsequently chosen for the implantation in recipient mice. One week prior to the implantation surgery, recipient mice are oophorectomized, and estradiol pellets are placed subcutaneously to sustain the survival and growth of lesions. On the day of surgery mice are anesthetized, and peritoneal cavity accessed through a small (1.5 cm) incision by the linea-alba. Fluorescently labeled implants are tweezed, briefly soaked in glue and attached to the peritoneal layer. Incisions are sutured, and animals left to recover for a couple of days. Fluorescence emitted by endometriotic implants is usually non-invasively monitored every 3 days for 4 weeks with an in vivo imaging system. Variations in the size of endometriotic implants can be estimated in real time by quantification of the mCherry signal and normalization against the initial time-point showing maximal fluorescence intensity.
Traditional preclinical rodents of models of endometriosis do not allow non-invasive monitoring of lesion in real time but rather allow evaluation of the effects of drugs assayed at the end point. This protocol allows one to track lesions in real time and is more useful to explore the therapeutic potential of drugs in preclinical models of endometriosis. The main limitation of the model thus generated is that non-invasive monitoring is not possible over long periods of time due to the episomal expression of Ad-virus.

Here, we present a protocol for live-imaging of fluorescently labeled human endometrial fragments grafted in mice. The method allows studying the effects of drugs of choice on endometriotic lesion size through monitoring and quantification of fluorescence emitted by the fluorescent reporter on real time

Nicht-invasive Überwachung der Größe der Läsion in einer heterologen Mausmodell der Endometriose

Here, we describe a protocol for the implementation of a heterologous mouse model in which progression of endometriosis can be assessed in real time ...

Development of a Novel Endometrioma-Simulating Mouse Model

Establishment of an Experimental Mouse Model of Endometrioma to Study its Related Infertility

Endometrioma (OMA), a subtype of endometriosis characterized by the formation of endometriotic cysts in the ovaries, affects 17-44% of individuals diagnosed with endometriosis. Women with OMA often experience compromised fertility, yet the exact mechanisms underlying OMA-associated infertility remain unclear. Notably, existing animal models simulate superficial peritoneal endometriosis (SUP) and deep infiltrating endometriosis (DIE), leaving a notable gap in research focused on OMA. In response to the gap of knowledge, this paper introduces a pioneering OMA-simulating mouse model and provides a comprehensive description of the techniques and procedures employed in the model. With a high success rate of 83% and ovarian lesion specificity, this model holds significant promise for advancing our understanding of OMA, particularly in the context of infertility. It offers a valuable platform for conducting targeted research into OMA-associated fertility challenges, potentially paving the way for improved diagnostic and therapeutic strategies in the field of reproductive medicine.

This study introduces a novel mouse model specifically designed to simulate endometrioma, a clinically significant subtype of endometriosis. By utilizing C57BL/6J mice, the model aims to elucidate the pathophysiological mechanisms underlying endometrioma-related infertility, offering a refined tool to bridge the current knowledge gap in reproductive medicine.

Endometrioma (OMA), a subtype of endometriosis characterized by the formation of endometriotic cysts in the ovaries, affects 17-44% of individuals ...

Establishing 3D Endometrial Organoids from the Mouse Uterus

Endometrial tissue lines the inner cavity of the uterus and is under the cyclical control of estrogen and progesterone. It is a tissue that is composed of luminal and glandular epithelium, a stromal compartment, a vascular network, and a complex immune cell population. Mouse models have been a powerful tool to study the endometrium, revealing critical mechanisms that control implantation, placentation, and cancer. The recent development of 3D endometrial organoid cultures presents a state-of-the-art model to dissect the signaling pathways that underlie endometrial biology. Establishing endometrial organoids from genetically engineered mouse models, analyzing their transcriptomes, and visualizing their morphology at a single-cell resolution are crucial tools for the study of endometrial diseases. This paper outlines methods to establish 3D cultures of endometrial epithelium from mice and describes techniques to quantify gene expression and analyze the histology of the organoids. The goal is to provide a resource that can be used to establish, culture, and study the gene expression and morphological characteristics of endometrial epithelial organoids.

This protocol describes methodologies to establish mouse endometrial epithelial organoids for gene expression and histological analyses.

Etablierung von 3D-Endometrium-Organoiden aus der Gebärmutter der Maus

Endometrial tissue lines the inner cavity of the uterus and is under the cyclical control of estrogen and progesterone. It is a tissue that is composed of ...

Optimized Thin Endometrium Mouse Model with Low Mortality and Minimal Adhesions

Establishing a Mouse Model of Thin Endometrium

Thin endometrium (TE) has been widely recognized as a critical cause of infertility. However, the pathogenesis of TE remains unclear, and satisfactory treatment options are still urgently needed. Several animal models of TE have been developed, but the mouse model involving abdominal surgery and injection of 95% ethanol presents a formidable challenge due to the high mortality rate and risk of intrauterine adhesions if not performed correctly. Here, we describe a detailed protocol that produces reliable and reproducible TE with a very low mortality rate and minimal intrauterine adhesions by injecting 95% ethanol into the mouse uterus with varying infusion times. The results showed that all of the mice successfully developed TE with infusion times ranging from 1-3 min, characterized by a typical reduction in endometrial thickness and the number of glands, as well as excessive endometrial fibrosis. These findings suggest that this mouse model is suitable for studying thin endometrium and can serve as a platform for developing future TE treatments.

This article describes a detailed protocol for producing a reliable and reproducible thin endometrium with a very low mortality rate and minimal intrauterine adhesions by injecting 95% ethanol into the mouse uterus within 1-3 min.

Thin endometrium (TE) has been widely recognized as a critical cause of infertility. However, the pathogenesis of TE remains unclear, and satisfactory ...

Modeling Metastatic Endometrial Cancer  Model: A Technique to Generate Endometrial Cancer with Lymph Node Metastasis in Rabbit Models

Use a number 11 scalpel blade to make a 2.5-centimeter incision, 1 centimeter cranial to the symphysis pubis through skin and subcutaneous tissue. Incise the rectus fascia and dissect the rectus muscles laterally to expose the underlying peritoneum. Confirm that the under surface is clear of bowel or other abdominal organs. Sharply enter the peritoneum to identify the urinary bladder and sweep a gloved finger superiorly, posteriorly, and laterally over the apex of the bladder to locate the uterine horns.
Extract the uterine horns through the abdominal incision to rest on the abdominal wall. Use a 3-0 braided absorbable suture to perform a single suture ligation of each uterine horn, approximately 1.5 to 2 centimeters distal to the cervices and just medial to the uterine arteries. Then, tie the sutures snugly to occlude the distal uterine horns.
To inoculate the myometrium with the previously prepared VX2 cell suspension, load a 1-milliliter syringe equipped with a 27 gauge needle with 1 milliliter of cells. Slowly inject 0.5 milliliters of the cell suspension over 1 minute into each uterine horn proximal to the suture site between the suture and the cervix. Then, apply pressure to the myometrial injection site for 30 seconds to minimize cell leakage. Inspect the injection and suture sites for hemostasis before placing the uterine horns back into the abdomen.

Modellierung des metastasierten Endometriumkarzinommodells: Eine Technik zur Erzeugung von Endometriumkarzinom mit Lymphknotenmetastasen in Kaninchenmodellen