Un merci spécial à Chris et Audrey Kassotis Bailey pour un examen critique de ce manuscrit et au Dr Scott Korte, Joseph Beeman, Alison Curfman, Paul Kimball, Bridget Neibreggue, Jacob Redel, Amy Schroder, Maija Steinberg, et Stacey Winkeler pour leur aide dans l&#39;optimisation de ce modèle dans notre laboratoire. Le financement a été fourni par la clinique Grant Biodetectives Formation (NIH T90) (KEP), Université du Missouri Life Sciences Research Programme de premier cycle des chances, MU Conseil de recherches, MU subventions pour la recherche et de conseil NIH R21HD056441 (SCN).

<ol>
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Steril. 93, 1615-1627 (2010).</li><li>Flores, I. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Molecular+profiling+of+experimental+endometriosis+identified+gene+expression+patterns+in+common+with+human+disease.">Molecular profiling of experimental endometriosis identified gene expression patterns in common with human disease.</a> Fertil. Steril. 87, 1180-1199 (2007).</li><li>Giudice, L. C., Kao, L. C. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Endometriosis.">Endometriosis.</a> Lancet. 364, 1789-1799 (2004).</li><li>Lin, Y. J., Lai, L. e. i., Y, H., Wing, L. 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E., Edwards, R. G. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Induction+of+superovulation+and+pregnancy+in+mature+mice+by+gonadotrophins.">Induction of superovulation and pregnancy in mature mice by gonadotrophins.</a> J. Endocrinol. 15, 374-384 (1957).</li><li>Wilson, E. D., Zarrow, M. X. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Comparison+of+superovulation+in+the+immature+mouse+and+rat.">Comparison of superovulation in the immature mouse and rat.</a> J. Reprod. Fertil. 3, 148-158 (1962).</li><li>Lee, B., Du, H., Taylor, H. S. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Experimental+murine+endometriosis+induces+DNA+methylation+and+altered+gene+expression+in+eutopic+endometrium.">Experimental murine endometriosis induces DNA methylation and altered gene expression in eutopic endometrium.</a> Biol. Reprod. 80, 79-85 (2009).</li><li>Somigliana, E. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Endometrial+ability+to+implant+in+ectopic+sites+can+be+prevented+by+interleukin-12+in+a+murine+model+of+endometriosis.">Endometrial ability to implant in ectopic sites can be prevented by interleukin-12 in a murine model of endometriosis.</a> Hum. Reprod. 14, 2944-2950 (1999).</li><li>Hirata, T. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Development+of+an+experimental+model+of+endometriosis+using+mice+that+ubiquitously+express+green+fluorescent+protein.">Development of an experimental model of endometriosis using mice that ubiquitously express green fluorescent protein.</a> Hum. Reprod. 20, 2092-2096 (2005).</li><li>Story, L., Kennedy, S. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Animal+studies+in+endometriosis:+a+review.">Animal studies in endometriosis: a review.</a> Ilar. J. 45, 132-138 (2004).</li><li>Cummings, A. M., Hedge, J. M., Birnbaum, L. S. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Effect+of+prenatal+exposure+to+TCDD+on+the+promotion+of+endometriotic+lesion+growth+by+TCDD+in+adult+female+rats+and+mice.">Effect of prenatal exposure to TCDD on the promotion of endometriotic lesion growth by TCDD in adult female rats and mice.</a> Toxicol. Sci. 52, 45-49 (1999).</li><li>Cummings, A. M., Metcalf, J. L. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Effects+of+estrogen,+progesterone,+and+methoxychlor+on+surgically+induced+endometriosis+in+rats.">Effects of estrogen, progesterone, and methoxychlor on surgically induced endometriosis in rats.</a> Fundam. Appl. Toxicol. 27, 287-290 (1995).</li><li>Sharpe-Timms, K. L. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Endometriotic+lesions+synthesize+and+secrete+a+haptoglobin-like+protein.">Endometriotic lesions synthesize and secrete a haptoglobin-like protein.</a> Biol. Reprod. 58, 988-994 (1998).</li><li>Yavuz, E., Oktem, M., Esinler, I., Toru, S. A., Zeyneloglu, H. B. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Genistein+causes+regression+of+endometriotic+implants+in+the+rat+model.">Genistein causes regression of endometriotic implants in the rat model.</a> Fertil. Steril. 88, 1129-1134 (2007).</li><li>Dmitrieva, N. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Endocannabinoid+involvement+in+endometriosis.">Endocannabinoid involvement in endometriosis.</a> Pain. 151, 703-710 (2010).</li><li>Efstathiou, J. A. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Nonsteroidal+antiinflammatory+drugs+differentially+suppress+endometriosis+in+a+murine+model.">Nonsteroidal antiinflammatory drugs differentially suppress endometriosis in a murine model.</a> Fertil. Steril. 83, 171-181 (2005).</li><li>Becker, C. M. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Endostatin+inhibits+the+growth+of+endometriotic+lesions+but+does+not+affect+fertility.">Endostatin inhibits the growth of endometriotic lesions but does not affect fertility.</a> Fertil. Steril. 84, 1144-1155 (2005).</li><li>Becker, C. M. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Short+synthetic+endostatin+peptides+inhibit+endothelial+migration+in+vitro+and+endometriosis+in+a+mouse+model.">Short synthetic endostatin peptides inhibit endothelial migration in vitro and endometriosis in a mouse model.</a> Fertil. Steril. 85, 71-77 (2006).</li><li>Sharpe-Timms, K. L. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Using+rats+as+a+research+model+for+the+study+of+endometriosis.">Using rats as a research model for the study of endometriosis.</a> Ann. N.Y. Acad. Sci. 955, 318-327 (2002).</li><li>Stilley, J. A., Woods-Marshall, R., Sutovsky, M., Sutovsky, P., Sharpe-Timms, K. L. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Reduced+Fecundity+in+Female+Rats+with+Surgically+Induced+Endometriosis+and+in+Their+Daughters:+A+Potential+Role+for+Tissue+Inhibitors+of+Metalloproteinase+1.">Reduced Fecundity in Female Rats with Surgically Induced Endometriosis and in Their Daughters: A Potential Role for Tissue Inhibitors of Metalloproteinase 1.</a> Biol. Reprod. 80, (2009).</li></ol>

Pas de conflits d&#39;intérêt déclarés.

Il ya plusieurs paramètres critiques qui doivent être notés dans l&#39;exercice de l&#39;induction chirurgicale de l&#39;endométriose chez la souris. Premièrement, l&#39;endométriose est une maladie oestrogènes dépendantes et en tant que telle cette chirurgie doit être réalisée chez les animaux intacts ou bien chez les animaux ayant subi une ovariectomie complétées avec des oestrogènes 20. Deuxièmement, la suture des biopsies endométriales à la cascade artérielle doit être effectuée avec u...

<table border="1"><tbody><tr><td> Nom du réactif / équipements </td><td> Société </td><td> Numéro de catalogue </td></tr><tr><td> Crayon de cire </td><td> Fisher </td><td> NC9954135 </td></tr><tr><td> Lame de verre </td><td> Fisher </td><td> 12-550-433 </td></tr><tr><td> Pipette </td><td> Fisher </td><td> S79383 </td></tr><tr><td> Microscope optique standard pour l&#39;évaluation des frottis vaginaux </td><td></td><td></td></tr><tr><td> La buprénorphine HCL C3 (CARJET) 10X1ml </td><td> Alimentation Butler Santé Animale </td><td> 022891 </td></tr><tr><td> Phosphate stérile saline tamponnée (PBS) </td><td> Gibco </td><td> 14040-117 </td></tr><tr><td> 10000 U / ml de pénicilline, 10.000 pg / ml de streptomycine dans le NaCl à 0,85% </td><td> Hyclone </td><td> SV30010 </td></tr><tr><td> L&#39;isoflurane </td><td> AbbottSanté Animale </td><td> 05260-05 </td></tr><tr><td> L&#39;isoflurane non réinhalation système d&#39;anesthésie </td><td></td><td></td></tr><tr><td> Recirculation pad chauffage à eau chaude </td><td></td><td></td></tr><tr><td> 30 ml gaine de seringues </td><td> Fisher </td><td> 14 à 823-16G </td></tr><tr><td> Poudre libre gants stériles </td><td> Fisherbrand </td><td> 19020558 </td></tr><tr><td> Pommade ophtalmique </td><td> Pharmaceuticals Major </td><td> 10033691 </td></tr><tr><td> Petite tondeuse électrique </td><td> Wahl </td><td> 9861-600 </td></tr><tr><td> Gommage chlorhexidine </td><td> Fisher </td><td> NC9863042 </td></tr><tr><td> Ethanol à 70% </td><td></td><td></td></tr><tr><td> Polylined champ stérile </td><td> Jetables Hôpital Busse </td><td> 696 </td></tr><tr><td> Taille 3 bistouri </td><td> Fisher </td><td> 22-079-657 </td></tr><tr><td> Numéro 10 lames de scalpel </td><td> Fisher </td><td> 22-079-681 </td></tr><tr><td> Petits ciseaux chirurgicaux </td><td> Roboz </td><td> RS-5850 </td></tr><tr><td> Petits dentelés semi-courbée pince </td><td> Roboz </td><td> RS-5135 </td></tr><tr><td> 5-0 soie noire tressée </td><td> Ethicon </td><td> K870H </td></tr><tr><td> Stérilisés en verre pyrex boîtes de Pétri </td><td> Corning </td><td> 70160-101 </td></tr><tr><td> 2 mm biopsie </td><td> Miltex </td><td> 33-31 </td></tr><tr><td> Gaze stérile </td><td> Kendall </td><td> 1806 </td></tr><tr><td> 6-0 suture noire en nylon monofilament Ethilon </td><td> Ethicon </td><td> 697G </td></tr><tr><td> Les conducteurs de seringues (en option) </td><td> Instruments de précision du monde </td><td> 500023 </td></tr><tr><td> 5-0 écru tresse couché vicryl de suture </td><td> Ethicon </td><td> J490G </td></tr><tr><td> 9mm Autoclip plaies clips </td><td> Becton Dickinson </td><td> 427631 </td></tr><tr><td> Autoclip applicateur &amp; Remover </td><td> Becton Dickinson </td><td> 427630 </td></tr><tr><td> Aiguille 23G </td><td> Becton Dickinson </td><td> 305193 </td></tr><tr><td> Seringue 1cc </td><td> Becton Dickson </td><td> 301025 </td></tr><tr><td> 5X se loupe (facultatif) </td><td> Fisher </td><td> 14-648-23 </td></tr><tr><td> 10% du formol tamponné </td><td> Fisher </td><td> SF100-4 </td></tr><tr><td> Étriers </td><td> Roboz </td><td> RS-6466 </td></tr><tr><td> Traitement / embedding cassettes </td><td> Fisher </td><td> 15 à 197-700A </td></tr><tr><td> Coussinets en mousse biopsie </td><td> Fisher </td><td> 22-038-222 </td></tr><tr><td> RNAqueous kit d&#39;isolement d&#39;ARN </td><td>Ambion </td><td> AM1912 </td></tr><tr><td> L&#39;azote liquide </td><td></td><td></td></tr><tr><td> Microcentrifugeuse bouchon rabattre à plat tube supérieur </td><td> Fisher </td><td> 02-681-240 </td></tr><tr><td> La kétamine (facultatif) </td><td> Simga </td><td> K4138 </td></tr><tr><td> Domitor (chlorhydrate de médétomidine) (facultatif) </td><td> Tocris </td><td> 2023 </td></tr><tr><td> Antisedan (atipamézole) (facultatif) </td><td> Sigma </td><td> A9611 </td></tr></tbody></table>

mouse model of surgically-induced endometriosis by auto-transplantation of uterine tissue

L&#39;endométriose est une maladie chronique, douloureuse dont l&#39;étiologie reste inconnue. Par ailleurs, le traitement de l&#39;endométriose peuvent exiger l&#39;enlèvement laparoscopique des lésions, et / ou chronique gestion pharmaceutique de la douleur et des symptômes d&#39;infertilité. Le coût associé à l&#39;endométriose a été estimé à 22 milliards de dollars par an dans le 1 États-Unis. Pour approfondir notre compréhension des mécanismes sous-jacents de cette maladie énigmatique, des modèles animaux ont été employées. Les primates se développent spontanément l&#39;endométriose et des modèles de primates conséquent ressemblent le plus à la maladie chez les femmes. Des modèles de rongeurs, cependant, sont plus rentables et facilement disponibles 2. Le modèle que nous décrivons ici implique un transfert autologue de tissu utérin au mésentère intestinal (Figure 1) et a été d&#39;abord développé chez le rat 3 et transféré plus tard à la souris 4. Le but du modèle de rongeur autologue de l&#39;endométriose chirurgicalement induite est d&#39;imiterla maladie chez les femmes. Nous et d&#39;autres ont déjà montré que le modèle gène modifié expression observée dans les lésions d&#39;endométriose chez les souris ou les rats qui miroirs observés chez les femmes avec la maladie 5,6. Un avantage de réaliser l&#39;intervention chez la souris est que l&#39;abondance des souches de souris transgéniques disponibles peuvent aider les chercheurs à déterminer le rôle des composants spécifiques important dans la création et la croissance de l&#39;endométriose. Un modèle alternatif dans lequel des fragments humains excisés endomètre sont introduits dans le péritoine de souris immunodéprimées est aussi largement utilisée, mais est limitée par l&#39;absence d&#39;un système immunitaire normal qui est pensé pour être important dans l&#39;endométriose 2,7. Surtout, le modèle de souris de l&#39;endométriose chirurgicalement induite est un modèle polyvalent qui a été utilisée pour étudier comment le système immunitaire 8, 9,10 hormones et des facteurs environnementaux affectent 11,12 endométriose ainsi que les effets de l&#39;endométriose sur Fertilité 13 et de la douleur 14.

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Mouse Model of Surgically-induced Endometriosis by Auto-transplantation of Uterine Tissue

Une description de l&#39;induction chirurgicale de l&#39;endométriose chez les souris et les rats par auto-transplantation de tissu utérin à la cascade de l&#39;artère mésentère intestinal.

Modèle murin d&#39;endométriose chirurgicalement induite par Auto-transplantation de tissu utérin

Endometriosis is a chronic, painful disease whose etiology remains unknown. Furthermore, treatment of endometriosis can require laparoscopic removal of ...

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28.3K vues.  University of Missouri. Une description de l'induction chirurgicale de l'endométriose chez les souris et les rats par auto-transplantation de tissu utérin à la cascade de l'artère mésentère intestinal.