Agradecimentos especiais a Chris Bailey Kassotis e Audrey para a revisão crítica do manuscrito e ao Dr. Scott Korte, Beeman Joseph, Curfman Alison, Kimball Paulo, Neibreggue Bridget, Redel Jacob, Schroder Amy, Steinberg Maija e Winkeler Stacey, o auxílio na otimização deste modelo em nosso laboratório. O financiamento foi fornecido pela Clínica Biodetectives Training Grant (NIH T90) (KEP), University of Missouri Life Sciences Research Graduação Programa de Oportunidades, Pesquisa MU Conselho, Research Board MU subvenções e R21HD056441 NIH (SCN).

<ol>
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Steril. 93, 1615-1627 (2010).</li><li>Flores, I. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Molecular+profiling+of+experimental+endometriosis+identified+gene+expression+patterns+in+common+with+human+disease.">Molecular profiling of experimental endometriosis identified gene expression patterns in common with human disease.</a> Fertil. Steril. 87, 1180-1199 (2007).</li><li>Giudice, L. C., Kao, L. C. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Endometriosis.">Endometriosis.</a> Lancet. 364, 1789-1799 (2004).</li><li>Lin, Y. J., Lai, L. e. i., Y, H., Wing, L. 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Existem vários parâmetros críticos que devem ser observadas durante a realização da indução cirúrgica de endometriose em camundongos. Primeiro, a endometriose é uma doença estrogênio dependente e, como tal, esta cirurgia deve ser realizada em animais intactos ou, alternativamente, em animais ovariectomizados suplementado com estrógenos 20. Segundo, suturar as biópsias endometriais para a cascata arterial deve ser realizada com extremo cuidado. Nós descobrimos que o uso de apenas dois nós relati...

<table border="1"><tbody><tr><td> Nome do reagente / equipamentos </td><td> Companhia </td><td> Número de catálogo </td></tr><tr><td> Lápis de cera </td><td> Pescador </td><td> NC9954135 </td></tr><tr><td> Lâmina de vidro </td><td> Pescador </td><td> 12-550-433 </td></tr><tr><td> Conta-gotas </td><td> Pescador </td><td> S79383 </td></tr><tr><td> Microscópio de luz padrão para avaliar manchas de citologia vaginal </td><td></td><td></td></tr><tr><td> Buprenorfina HCL c3 (CARJET) 10X1ml </td><td> Butler Abastecimento da Saúde Animal </td><td> 022891 </td></tr><tr><td> Fosfato estéril salina tamponada (PBS) </td><td> Gibco </td><td> 14040-117 </td></tr><tr><td> 10.000 U / ml de penicilina, estreptomicina 10.000 mg / ml em NaCl 0,85% </td><td> Hyclone </td><td> SV30010 </td></tr><tr><td> Isoflurano </td><td> AbbottSaúde Animal </td><td> 05260-05 </td></tr><tr><td> Isoflurano não-reinalação sistema anestésico </td><td></td><td></td></tr><tr><td> Recirculação almofada de aquecimento de água quente </td><td></td><td></td></tr><tr><td> 30 bainha seringa ml </td><td> Pescador </td><td> 14-823-16G </td></tr><tr><td> Pó livre luvas estéreis </td><td> Fisherbrand </td><td> 19020558 </td></tr><tr><td> Pomada oftálmica </td><td> Pharmaceuticals principais </td><td> 10033691 </td></tr><tr><td> Pequenas tesouras elétricas </td><td> Wahl </td><td> 9861-600 </td></tr><tr><td> Matagal clorexidina </td><td> Pescador </td><td> NC9863042 </td></tr><tr><td> Etanol 70% </td><td></td><td></td></tr><tr><td> Campo estéril Polylined </td><td> Hospital Descartáveis ​​Busse </td><td> 696 </td></tr><tr><td> Tamanho 3 bisturi </td><td> Pescador </td><td> 22-079-657 </td></tr><tr><td> Número de 10 lâminas de bisturi </td><td> Pescador </td><td> 22-079-681 </td></tr><tr><td> Pequenas tesouras cirúrgicas </td><td> Roboz </td><td> RS-5850 </td></tr><tr><td> Pequenas dentadas semi-curvada forceps </td><td> Roboz </td><td> RS-5135 </td></tr><tr><td> 5-0 de sutura de seda preta trançada </td><td> Ethicon </td><td> K870H </td></tr><tr><td> Esterilizado pirex de vidro placas de Petri </td><td> Corning </td><td> 70160-101 </td></tr><tr><td> 2 milímetros biópsia com punch </td><td> Miltex </td><td> 33-31 </td></tr><tr><td> Gaze estéril </td><td> Kendall </td><td> 1806 </td></tr><tr><td> 6-0 preto sutura de monofilamento nylon ethilon </td><td> Ethicon </td><td> 697G </td></tr><tr><td> Drivers de agulha (opcional) </td><td> Precision Instruments mundo </td><td> 500023 </td></tr><tr><td> 5-0 undyed trançado revestido vicryl sutura </td><td> Ethicon </td><td> J490G </td></tr><tr><td> 9 milímetros Autoclip ferida clips </td><td> Becton Dickinson </td><td> 427631 </td></tr><tr><td> Autoclip aplicador e removedor </td><td> Becton Dickinson </td><td> 427630 </td></tr><tr><td> Agulha 23G </td><td> Becton Dickinson </td><td> 305193 </td></tr><tr><td> Seringa 1cc </td><td> Becton Dickson </td><td> 301025 </td></tr><tr><td> Estande de vidro 5X de aumento (opcional) </td><td> Pescador </td><td> 14-648-23 </td></tr><tr><td> 10% de formalina tamponada </td><td> Pescador </td><td> SF100-4 </td></tr><tr><td> Calibrador </td><td> Roboz </td><td> RS-6466 </td></tr><tr><td> Processamento / incorporação cassetes </td><td> Pescador </td><td> 15-197-700A </td></tr><tr><td> Almofadas de espuma de biópsia </td><td> Pescador </td><td> 22-038-222 </td></tr><tr><td> Kit de isolamento RNAqueous RNA </td><td>Ambion </td><td> AM1912 </td></tr><tr><td> Nitrogênio líquido </td><td></td><td></td></tr><tr><td> Microcentrífuga tirar tampa do tubo topo plano </td><td> Pescador </td><td> 02-681-240 </td></tr><tr><td> Ketamina (opcional) </td><td> Simga </td><td> K4138 </td></tr><tr><td> Domitor (cloridrato de medetomidina) (opcional) </td><td> Tocris </td><td> 2023 </td></tr><tr><td> Antisedan (atipamezol) (opcional) </td><td> Sigma </td><td> A9611 </td></tr></tbody></table>

mouse model of surgically-induced endometriosis by auto-transplantation of uterine tissue

A endometriose é uma doença crônica e dolorosa cuja etiologia permanece desconhecida. Além disso, o tratamento da endometriose pode exigir a remoção laparoscópica de lesões e / ou gestão farmacêutica crônico de dor e os sintomas de infertilidade. O custo associado com endometriose tem sido estimado em 22 bilhões de dólares por ano nos Estados Unidos 1. Para continuar a nossa compreensão dos mecanismos subjacentes a esta doença enigmática, modelos animais têm sido empregados. Primatas espontaneamente desenvolver endometriose e, portanto, modelos de primatas mais semelhantes ao da doença em mulheres. Modelos de roedores, no entanto, são mais rentáveis ​​e prontamente disponíveis 2. O modelo que descrevemos aqui envolve uma transferência autólogo de tecido uterino ao mesentério intestinal (Figura 1) e foi desenvolvido pela primeira vez no rato 3 e mais tarde transferido para o mouse 4. O objetivo do modelo de roedor autólogo de endometriose cirurgicamente induzida é imitarda doença em mulheres. Nós e os outros já haviam demonstrado que o padrão de expressão alterada do gene observada em lesões de endometriose de camundongos ou ratos espelhos que a observada em mulheres com a doença 5,6. Uma vantagem de realizar a cirurgia em ratos é que a abundância de linhagens de camundongos transgênicos disponíveis podem ajudar os pesquisadores a determinar o papel de componentes específicos importantes no estabelecimento e crescimento da endometriose. Um modelo alternativo no qual excisadas humana fragmentos do endométrio são introduzidos no peritônio de ratos imunocomprometidos também é amplamente utilizada, mas é limitado pela falta de um sistema imunológico normal que é pensado para ser importante na endometriose 2,7. Importante, o modelo do rato da endometriose induzida cirurgicamente é um modelo versátil, que tem sido usado para estudar como o sistema imunológico 8, 9,10 hormônios e fatores ambientais afetam 11,12 endometriose, bem como os efeitos da endometriose sobre fertility 13 e 14 a dor.

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Mouse Model of Surgically-induced Endometriosis by Auto-transplantation of Uterine Tissue

Uma descrição da indução cirúrgica de endometriose em camundongos e ratos por auto-transplante de tecido do útero para a cascata arterial do mesentério intestinal.

Modelo do rato de endometriose cirurgicamente induzida pela Auto-transplante de tecido uterino

Endometriosis is a chronic, painful disease whose etiology remains unknown. Furthermore, treatment of endometriosis can require laparoscopic removal of ...

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28.3K visualizações.  University of Missouri. Uma descrição da indução cirúrgica de endometriose em camundongos e ratos por auto-transplante de tecido do útero para a cascata arterial do mesentério intestinal.