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Proteomic Sample Preparation from Formalin Fixed and Paraffin Embedded Tissue

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09:20 min

September 2nd, 2013

September 2nd, 2013

27,343 Views

0:05

Title

1:46

Preparation of Tissue Slices for Microdissection and Sample Lysis

3:20

Sample Processing

4:51

Quantification of the Peptides

5:38

Fractionation of the Lys-C and Tryptic Peptides

7:29

Results: Peptide Quantification

8:21

Conclusion

Archival formalin fixed and paraffin embedded (FFPE) clinical samples are valuable material for investigation of diseases. Here we demonstrate a sample preparation workflow allowing in-depth proteomic analysis of microdissected FFPE tissue.

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