Epithelialen Zellwiederbevölkerung und Herstellung von Nagetier extrazellulären Matrix Scaffolds für Nierengewebe Entwicklung

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15.9K Views

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09:43 min

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August 10th, 2015

DOI :

10.3791/53271-v

August 10th, 2015

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Joseph S. Uzarski¹^,², Jimmy Su¹^,²^,³^,⁴, Yan Xie¹^,², Zheng J. Zhang¹^,², Heather H. Ward⁵, Angela Wandinger-Ness⁶, William M. Miller⁷^,⁸, Jason A. Wertheim¹^,²^,³^,⁴^,⁸^,⁹

¹Comprehensive Transplant Center, Feinberg School of Medicine, Northwestern University, ²Department of Surgery, Feinberg School of Medicine, Northwestern University, ³Department of Biomedical Engineering, Northwestern University, ⁴Simpson Querrey Institute for BioNanotechnology in Medicine, Northwestern University, ⁵Department of Internal Medicine, University of New Mexico HSC, ⁶Department of Pathology, University of New Mexico HSC, ⁷Department of Chemical and Biological Engineering, Northwestern University, ⁸Chemistry of Life Processes Institute, Northwestern University, ⁹Department of Surgery, Jesse Brown VA Medical Center

Zusammenfassung

This protocol describes decellularization of Sprague Dawley rat kidneys by antegrade perfusion of detergents through the vasculature, producing acellular renal extracellular matrices that serve as templates for repopulation with human renal epithelial cells. Recellularization and use of the resazurin perfusion assay to monitor growth is performed within specially-designed perfusion bioreactors.

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