Results: Assembly of WSCP Apoproteins Using Water in Oil Emersions
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Conclusion
Transkript
The overall goal of this technique is to enable incorporation of water-insoluble chlorophylls into the binding sites of water-soluble proteins. This method enables assembly of chlorophylls with recombinant proteins, which allows rigorous studies o
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This manuscript describes a simple and high-throughput method for assembling water-soluble proteins with hydrophobic pigments that is based on water-in-oil emulsions. We demonstrate the effectiveness of the method in the assembly of native chlorophylls with four variants of recombinant water-soluble-chlorophyll binding proteins (WSCPs) of Brassica plants expressed in E. coli.