Reverse Transfection of siRNA and Seeding of HeLa 229 Cells
4:25
Infection of Reverse Transfected Cells
5:39
Addition of BlaM Substrate to Determine Level of Translocation
8:23
Results: FRET Measurements of Coxiella burnetti Effector Translocation Efficiency in siRNA Silenced HeLa Cells
9:37
Conclusion
Transkript
The overall goal of this experimental procedure is to examine the impact of specific host processes on the ability of bacterial pathogens to translocate effector proteins. This method can help answer key questions in the field of cellular microbio
Sign in or start your free trial to access this content
Investigating the interactions between bacterial pathogens and their hosts is an important area of biological research. Here, we describe the necessary techniques to measure effector translocation by Coxiella burnetii during siRNA gene silencing using BlaM substrate.