Single Cell Suspension Staining for Flow Cytometric Analysis
5:49
Results: Representative Microglia Phagocytic Function after LPS Challenge in Pup and Adult Mice
6:30
Conclusion
Transkript
The overall goal of this procedure is to assess the phagocytic function of retinal microglia in vivo. This method can help answer key questions in the ophthalmology field, such as whether certain compounds alter microglia phagocytic function in a
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Microglial phagocytosis is critical for the maintenance of tissue homeostasis and inadequate phagocytic function has been implicated in pathology. However, assessing microglia function in vivo is technically challenging. We have developed a simple but robust technique for precisely monitoring and quantifying the phagocytic potential of microglia in a physiological setting.