Results: Measuremnt of Mitochondrial Membrane Potential and Calcium in Digitonin Permeabilized 143B Cells
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Conclusion
Transkript
The overall goal of this procedure is to provide a simple method for simultaneously measuring mitochondrial calcium uptake and mitochondrial membrane potential in live cells using confocal microscopy. This method can help to answer key questions i
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Mitochondria can utilize the electrochemical potential across their inner membrane (ΔΨm) to sequester calcium (Ca2+), allowing them to shape cytosolic Ca2+ signaling within the cell. We describe a method for simultaneously measuring mitochondria Ca2+ uptake and ΔΨm in live cells using fluorescent dyes and confocal microscopy.