Detection of the Mitochondrial Membrane Potential (MMP)
6:29
Confocal Laser Scanning Fluorescence Microscope
8:34
Statistical Analysis
9:48
Results: Detection of Mitochondrial Membrane Potential via Normal Fluorescent Microscopy and Confocal Laser Scanning Microscopy
11:53
Conclusion
Transkript
This method can help answer the key questions in the cytobiological field including understanding the apoptosis and mitochondria dysfunction. The main advantage of this technique is that the investigators can easily and properly conduct the real-t
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Here we present a detailed protocol for the application of rhodamine 123 to identify the mitochondrial membrane potential (MMP) and study CLIC4 knockdown-induced HN4 cell apoptosis in vitro. Under common fluorescence microscope and confocal laser scanning fluorescence microscope, the real-time change of the MMP was recorded.