Assessment of Binding Between Notch Receptor and Trans-ligands
1:47
Aggregation Between Signal-sending and Signal-receiving Cells
3:11
Evaluation of Inhibition of Binding Between Notch and Trans-ligands by Cis-ligands
4:15
Results: Faithful Analysis of Notch Signaling
5:30
Conclusion
Transkript
The overall goal of this aggregation assay is to assess the binding of Notch receptor with trans-ligands and its inhibition by cis-ligands. This method can help answer a key question, How will genetic or pharmacological factor affect the binding o
Sign in or start your free trial to access this content
Complexity of in vivo systems makes it difficult to distinguish between the activation and inhibition of Notch receptor by trans- and cis-ligands, respectively. Here, we present a protocol based on in vitro cell-aggregation assays for qualitative and semi-quantitative evaluation of the binding of Drosophila Notch to trans-ligands vs cis-ligands.