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JoVE Journal

Developmental Biology

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Methods to Examine the Lymph Gland and Hemocytes in Drosophila Larvae

12.6K Views

11:49 min

November 28th, 2016

November 28th, 2016

12,638 Views

0:05

Title

0:44

Hemolymph Collection to Determine Concentration

1:45

Hemolymph Collection and Immunochemistry

4:32

Larval Lymph Gland Dissection

6:31

Antibody Staining of Larval Lymph Glands

8:32

Mounting Lymph Gland Tissue for Microscopy

9:32

Results: Quantification and Imaging of Hemocytes, and Imaging of Dissected and Immunostained Lymph Gland Tissue

11:19

Conclusion

Transkript

The overall goal of these procedures, is to analyze various components of the Drosophila hematopoietic system with an emphasis on lymph gland dissection and mounting for immunohistochemistry. These methods can help answer key questions in the Dros

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Drosophila and mammalian hematopoietic systems share many common features, making Drosophila an attractive genetic model to study hematopoiesis. Here we demonstrate dissection and mounting of the major larval hematopoietic organ for immunohistochemistry. We also describe methods to assay various larval hematopoietic compartments including circulating hemocytes and sessile crystal cells.

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