Results: Study of the Lysosomal Functional Activity Using a pH Sensitive Dye
5:38
Conclusion
Transkript
The overall goal of this procedure is to assess a relative loss of lysosomal acidity in the intestinal lysosomes of C.elegans. This method can help answer key questions in the field of autophagy by indirectly providing information about lysosomal
Sign in or start your free trial to access this content
A step-by-step guide to probe loss of lysosomal acidity in the intestine of C. elegans using the pH-sensitive vital dye 5(6)-carboxy-2',7'-dichlorofluorescein diacetate (cDCFDA)