Imaging Analysis and Algorithm for Ratiometric Intensity-based Quantification of Photoactivation Efficiency
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Results: Maximizing Photoactivation Efficiency
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Conclusion
Transkript
We present here a protocol that involves genetically coupled spectrally distinct photo-activatable and fluorescent proteins.These internal rulers permit quantification of the PAFP fraction that is photo activated to be fluorescent.Thus far, no met
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Here, we present a protocol that involves genetically coupled spectrally distinct photoactivatable and fluorescent proteins. These fluorescent protein chimeras permit quantification of the PA-FP fraction that is photoactivated to be fluorescent, i.e., the photoactivation efficiency. The protocol reveals that different modes of photoactivation yield different photoactivation efficiencies.