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JoVE Journal

Biochemistry

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Dual DNA Rulers to Study the Mechanism of Ribosome Translocation with Single-Nucleotide Resolution

6.2K Views

10:27 min

July 8th, 2019

July 8th, 2019

6,200 Views

0:04

Title

0:43

Sample Preparation

4:23

Magnetic and Force Measurements

8:04

Results: Dual DNA Ruler Assay and Probing Translocation

9:51

Conclusion

Transkript

The dual DNA ruler assay can provide mechanistic insights for ribosome translocation and probe other nucleic acid displacements. Our dual DNA ruler assay is currently the only method that can objectively probe both the entrance and exit sides of m

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Dual DNA ruler assay is developed to determine the mRNA position during ribosome translocation, which relies on the dissociation forces of the formed DNA-mRNA duplexes. With single-nucleotide resolution and capability of reaching both ends of mRNA, it can provide mechanistic insights for ribosome translocation and probe other nucleic acid displacements.

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